Publication detail

Bioinformatic Tools for Genotyping of Klebsiella pneumoniae Isolates

NYKRÝNOVÁ, M. MADĚRÁNKOVÁ, D. BEZDÍČEK, M. LENGEROVÁ, M. ŠKUTKOVÁ, H.

Original Title

Bioinformatic Tools for Genotyping of Klebsiella pneumoniae Isolates

English Title

Bioinformatic Tools for Genotyping of Klebsiella pneumoniae Isolates

Type

conference paper

Language

en

Original Abstract

Nowadays we use multiple molecular typing methods capable of distinguishing bacteria strains. However, a majority of these methods are time-consuming, and associated costs are not negligible. Also, reproducibility of typing results is questionable. Therefore, we propose new typing methodology based on bioinformatics. In this paper, we present an algorithm that is at the core of the new method. It consists of multiple steps, such as measuring the quality of input data, identification of genes having high diversity and analysis of results employing clustering and phylogenetic trees. Obtained results are then compared with results from the mini-MLST method.

English abstract

Nowadays we use multiple molecular typing methods capable of distinguishing bacteria strains. However, a majority of these methods are time-consuming, and associated costs are not negligible. Also, reproducibility of typing results is questionable. Therefore, we propose new typing methodology based on bioinformatics. In this paper, we present an algorithm that is at the core of the new method. It consists of multiple steps, such as measuring the quality of input data, identification of genes having high diversity and analysis of results employing clustering and phylogenetic trees. Obtained results are then compared with results from the mini-MLST method.

Keywords

Bacterial strains, Genes, Genotyping, Klebsiella pneumoniae, Mini-MLST

Released

01.01.2019

ISBN

978-3-319-91210-3

Book

Information Technology in Biomedicine

Pages from

419

Pages to

428

Pages count

10

URL

BibTex


@inproceedings{BUT150417,
  author="Markéta {Nykrýnová} and Denisa {Maděránková} and Helena {Škutková} and Matěj {Bezdíček} and Martina {Lengerová}",
  title="Bioinformatic Tools for Genotyping of Klebsiella pneumoniae Isolates",
  annote="Nowadays we use multiple molecular typing methods capable of distinguishing bacteria strains. However, a majority of these methods are time-consuming, and associated costs are not negligible. Also, reproducibility of typing results is questionable. Therefore, we propose new typing methodology based on bioinformatics. In this paper, we present an algorithm that is at the core of the new method. It consists of multiple steps, such as measuring the quality of input data, identification of genes having high diversity and analysis of results employing clustering and phylogenetic trees. Obtained results are then compared with results from the mini-MLST method.",
  booktitle="Information Technology in Biomedicine",
  chapter="150417",
  doi="10.1007/978-3-319-91211-0_37",
  howpublished="online",
  year="2019",
  month="january",
  pages="419--428",
  type="conference paper"
}