Publication detail

Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

SLANINOVÁ, E. SEDLÁČEK, P. MRAVEC, F. MÜLLEROVÁ, L. SAMEK, O. KOLLER, M. HESKO, O. KUČERA, D. MÁROVÁ, I. OBRUČA, S.

Original Title

Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

English Title

Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

Type

journal article in Web of Science

Language

en

Original Abstract

Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments.

English abstract

Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments.

Keywords

Polyhydroxyalkanoates Cupriavidus necator UV radiation Turbidity Integrating sphere Nephelometry

Released

19.01.2018

Publisher

Springer

Location

Springer Berlin Heidelberg

Pages from

1923

Pages to

1931

Pages count

9

URL

BibTex


@article{BUT145891,
  author="Eva {Slaninová} and Petr {Sedláček} and Filip {Mravec} and Lucie {Müllerová} and Ota {Samek} and Ondrej {Hesko} and Dan {Kučera} and Ivana {Márová} and Stanislav {Obruča}",
  title="Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation",
  annote="Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments.",
  address="Springer",
  chapter="145891",
  doi="10.1007/s00253-018-8760-8",
  howpublished="online",
  institution="Springer",
  number="4",
  volume="102",
  year="2018",
  month="january",
  pages="1923--1931",
  publisher="Springer",
  type="journal article in Web of Science"
}