Publication detail

Interactions of hyaluronan with oppositely charged surfactants in very diluted solutions in water

PILGROVÁ, T. VENEROVÁ, T. MRAVEC, F. PEKAŘ, M.

Original Title

Interactions of hyaluronan with oppositely charged surfactants in very diluted solutions in water

English Title

Interactions of hyaluronan with oppositely charged surfactants in very diluted solutions in water

Type

journal article in Web of Science

Language

en

Original Abstract

The phase behavior of aqueous systems containing hyaluronan, at concentrations between 2 to 100 mg/L, and oppositely charged surfactants was investigated. A fluorescence probe technique revealed the formation of micellar structures on the hyaluronan in homogeneous systems well below the surfactant standard, critical, micellar concentration. Moreover, regions of gel-phase separation were revealed. A detailed phase diagram was, thus, constructed in the very diluted region and the hyaluronan concentration was found to be the main parameter controlling the phase behavior, in contrast to the charge ratio. The stability of hyaluronan-surfactant aggregates in the homogeneous systems while in storage at 4 °C (up to three months), against dilution, salt addition and on heating-cooling (between 10 and 50 °C) was also investigated. The aggregates were stable while in storage or upon increasing and decreasing the temperature. The dilution of hyaluronan-surfactant complexes or the addition of 0.15 M NaCl led to their disintegration. Finally, systems prepared in a 0.15 M NaCl solution showed that interactions are suppressed and no aggregation below the standard critical micellar concentration was observed.

English abstract

The phase behavior of aqueous systems containing hyaluronan, at concentrations between 2 to 100 mg/L, and oppositely charged surfactants was investigated. A fluorescence probe technique revealed the formation of micellar structures on the hyaluronan in homogeneous systems well below the surfactant standard, critical, micellar concentration. Moreover, regions of gel-phase separation were revealed. A detailed phase diagram was, thus, constructed in the very diluted region and the hyaluronan concentration was found to be the main parameter controlling the phase behavior, in contrast to the charge ratio. The stability of hyaluronan-surfactant aggregates in the homogeneous systems while in storage at 4 °C (up to three months), against dilution, salt addition and on heating-cooling (between 10 and 50 °C) was also investigated. The aggregates were stable while in storage or upon increasing and decreasing the temperature. The dilution of hyaluronan-surfactant complexes or the addition of 0.15 M NaCl led to their disintegration. Finally, systems prepared in a 0.15 M NaCl solution showed that interactions are suppressed and no aggregation below the standard critical micellar concentration was observed.

Keywords

hyaluronan; interactions; surfactants

Released

01.02.2018

Pages from

241

Pages to

249

Pages count

9

URL

BibTex


@article{BUT145670,
  author="Tereza {Pilgrová} and Tereza {Venerová} and Filip {Mravec} and Miloslav {Pekař}",
  title="Interactions of hyaluronan with oppositely charged surfactants in very
diluted solutions in water",
  annote="The phase behavior of aqueous systems containing hyaluronan, at concentrations between 2 to 100 mg/L, and oppositely charged surfactants was investigated. A fluorescence probe technique revealed the formation of micellar structures on the hyaluronan in homogeneous systems well below the surfactant standard, critical, micellar concentration. Moreover, regions of gel-phase separation were revealed. A detailed phase diagram was, thus, constructed in the very diluted region and the hyaluronan concentration was found to be the main parameter controlling the phase behavior, in contrast to the charge ratio. The stability of hyaluronan-surfactant aggregates in the homogeneous systems while in storage at 4 °C (up to three months), against dilution, salt addition and on heating-cooling (between 10 and 50 °C) was also investigated. The aggregates were stable while in storage or upon increasing and decreasing the temperature. The dilution of hyaluronan-surfactant complexes or the addition of 0.15 M NaCl led to their disintegration. Finally, systems prepared in a 0.15 M NaCl solution showed that interactions are suppressed and no aggregation below the standard critical micellar concentration was observed.",
  chapter="145670",
  doi="10.1016/j.ijbiomac.2018.01.176",
  howpublished="print",
  number="1",
  volume="112",
  year="2018",
  month="february",
  pages="241--249",
  type="journal article in Web of Science"
}