Publication detail

The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU

HARTINGER, J. VESELÝ, P. NETÍKOVÁ, I. MATOUŠKOVÁ, E. PETRUŽELKA, L.

Original Title

The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. Materials and Methods: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. Results: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. Conclusion: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment.

Keywords

Hand-foot syndrome; cytidine; uridine; thymidine; deoxycytidine; 5-FU; keratinocytes

Authors

HARTINGER, J.; VESELÝ, P.; NETÍKOVÁ, I.; MATOUŠKOVÁ, E.; PETRUŽELKA, L.

Released

1. 5. 2015

ISBN

0250-7005

Periodical

ANTICANCER RESEARCH

Year of study

35

Number

3

State

Hellenic Republic

Pages from

1303

Pages to

1310

Pages count

8

BibTex

@article{BUT142519,
  author="Jan {Hartinger} and Pavel {Veselý} and Irena {Štenglová Netíková} and Eva {Matoušková} and Luboš {Petruželka}",
  title="The Protective Effect of Pyrimidine Nucleosides on Human HaCaT Keratinocytes Treated with 5-FU",
  journal="ANTICANCER RESEARCH",
  year="2015",
  volume="35",
  number="3",
  pages="1303--1310",
  issn="0250-7005"
}