Publication detail

THE EFFECT OF HEAVY METAL IONS ON STAPHYLOCOCCUS AUREUS

CHUDOBOVA, D. DOSTÁLOVÁ, S. RUTTKAY-NEDECKÝ, B. MERLOS RODRIGO, M. ADAM, V. KIZEK, R.

Original Title

THE EFFECT OF HEAVY METAL IONS ON STAPHYLOCOCCUS AUREUS

Type

conference paper

Language

English

Original Abstract

Our objective was to determine the effect of heavy metal ions on resistant strains of Gram-positive bacterial strain of Staphylococcus aureus using mass spectrometry. The resistant strains of S. aureus were prepared using the nitrate solutions of metals (Ag, Cu, Cd, Zn and Pb). MALDI-TOF mass spectrometry was used for observation the changes in the protein composition in the cell wall and also for the determination and identification of the strains using the database MALDI Biotyper. Results obtained from analysis with resistant strains were compared with sensitive control strain of S. aureus. We observed alterations in S. aureus protein composition pointing at resistance development under influence of heavy metals ions. Our results develop the possible option of analysis of resistant strains and may serve as a support for the monitoring of changes in genetic information in resistant strains.

Keywords

S. aureus; resistance; heavy metals; mass spectrometry

Authors

CHUDOBOVA, D.; DOSTÁLOVÁ, S.; RUTTKAY-NEDECKÝ, B.; MERLOS RODRIGO, M.; ADAM, V.; KIZEK, R.

Released

1. 12. 2013

ISBN

978-80-7375-908-7

Book

MendelNet 2013

Pages from

743

Pages to

747

Pages count

5

BibTex

@inproceedings{BUT142422,
  author="Dagmar {chudobova} and Simona {Dostálová} and Branislav {Ruttkay-Nedecký} and Miguel Ángel {Merlos Rodrigo} and Vojtěch {Adam} and René {Kizek}",
  title="THE EFFECT OF HEAVY METAL IONS ON STAPHYLOCOCCUS AUREUS",
  booktitle="MendelNet 2013",
  year="2013",
  pages="743--747",
  isbn="978-80-7375-908-7"
}