Publication detail
Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells
BAIAZITOVA, L. ČMIEL, V. SKOPALÍK, J. SVOBODA, O. PROVAZNÍK, I.
Original Title
Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells
Type
conference paper
Language
English
Original Abstract
Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.
Keywords
Fluorescence lifetime; FLIM; cardiomyocyte; stromal cell; image segmentation
Authors
BAIAZITOVA, L.; ČMIEL, V.; SKOPALÍK, J.; SVOBODA, O.; PROVAZNÍK, I.
Released
28. 8. 2017
Location
Kos island, Greece
ISBN
978-0-9928626-7-1
Book
2017 25th European Signal Processing Conference (EUSIPCO)
ISBN
2219-5491
Periodical
European Signal Processing Conference EUSIPCO
State
United States of America
Pages from
439
Pages to
443
Pages count
5
URL
BibTex
@inproceedings{BUT138959,
author="Larisa {Chmelíková} and Vratislav {Čmiel} and Josef {Skopalík} and Ondřej {Svoboda} and Valentýna {Provazník}",
title="Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells",
booktitle="2017 25th European Signal Processing Conference (EUSIPCO)",
year="2017",
journal="European Signal Processing Conference EUSIPCO",
pages="439--443",
address="Kos island, Greece",
doi="10.23919/EUSIPCO.2017.8081245",
isbn="978-0-9928626-7-1",
issn="2219-5491",
url="https://ieeexplore.ieee.org/document/8081245"
}