Publication detail

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

BAIAZITOVA, L. ČMIEL, V. SKOPALÍK, J. SVOBODA, O. PROVAZNÍK, I.

Original Title

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

English Title

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Type

conference paper

Language

en

Original Abstract

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

English abstract

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

Keywords

Fluorescence lifetime; FLIM; cardiomyocyte; stromal cell; image segmentation

Released

28.08.2017

Location

Kos island, Greece

ISBN

978-0-9928626-7-1

Book

2017 25th European Signal Processing Conference (EUSIPCO)

Pages from

439

Pages to

443

Pages count

5

URL

https://ieeexplore.ieee.org/document/8081245

BibTex 


@inproceedings{BUT138959,
  author="Larisa {Chmelíková} and Vratislav {Čmiel} and Josef {Skopalík} and Ondřej {Svoboda} and Ivo {Provazník}",
  title="Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells",
  annote="Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.",
  booktitle="2017 25th European Signal Processing Conference (EUSIPCO)",
  chapter="138959",
  doi="10.23919/EUSIPCO.2017.8081245",
  howpublished="online",
  year="2017",
  month="august",
  pages="439--443",
  type="conference paper"
}