Publication detail

Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

ADAMCOVÁ, Z. MRAVEC, F. PEKAŘ, M.

Original Title

Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

English Title

Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

Type

abstract

Language

en

Original Abstract

This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.

English abstract

This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.

Keywords

FRET, perylene, fluorescein, cationic micelles

Released

30.05.2017

Publisher

Masaryk University

Location

Brno

ISBN

978-80-210-8580-0

Book

Book of abstracts

Edition number

1

Pages from

46

Pages to

46

Pages count

84

BibTex


@misc{BUT138433,
  author="Zuzana {Adamcová} and Filip {Mravec} and Miloslav {Pekař}",
  title="Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer",
  annote="This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.",
  address="Masaryk University",
  booktitle="Book of abstracts",
  chapter="138433",
  howpublished="electronic, physical medium",
  institution="Masaryk University",
  year="2017",
  month="may",
  pages="46--46",
  publisher="Masaryk University",
  type="abstract"
}