Publication detail

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

VANĚK, M. SZOTKOWSKI, M. MRAVEC, F. HÁRONIKOVÁ, A. MÁROVÁ, I.

Original Title

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

English Title

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

Type

abstract

Language

en

Original Abstract

Carotenoids are one of the most abundant natural pigment and they have great potential for wide industrial utilization in food and feed industry, cosmetics, pharmacy and even electronics.Carotenoids can be obtained by means of biotechnological production using so-called red yeasts strains while utilizing waste substrates. Red yeast Cystofilobasidium capitatum CCY 10-1-2 autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells.Yeasts were cultivated in 4L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and RP-HPLC/PDA determination of pigments. To compare FLIM images and coupled data (obtained by CLSM) with model systems, FLIM (fluorescence lifetime imaging) analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, an with constant addition of beta- carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoids:ergosterol clusters in different compartments of yeast cell. For more information about pigment forms, their changes during yeast growth and potential physiological significance, additional investigations should be performed

English abstract

Carotenoids are one of the most abundant natural pigment and they have great potential for wide industrial utilization in food and feed industry, cosmetics, pharmacy and even electronics.Carotenoids can be obtained by means of biotechnological production using so-called red yeasts strains while utilizing waste substrates. Red yeast Cystofilobasidium capitatum CCY 10-1-2 autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells.Yeasts were cultivated in 4L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and RP-HPLC/PDA determination of pigments. To compare FLIM images and coupled data (obtained by CLSM) with model systems, FLIM (fluorescence lifetime imaging) analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, an with constant addition of beta- carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoids:ergosterol clusters in different compartments of yeast cell. For more information about pigment forms, their changes during yeast growth and potential physiological significance, additional investigations should be performed

Keywords

red yeasts, FLIM, CLSM

Released

10.05.2016

Publisher

Institute of Chemistry, Slovak Academy of Sciences, Bratislava

Location

Bratislava

ISBN

1336-4839

Book

43rd Annual Conference on Yeasts - Book of Abstracts

Edition

1

Pages from

54

Pages to

54

Pages count

1

BibTex


@misc{BUT133224,
  author="Martin {Vaněk} and Martin {Szotkowski} and Filip {Mravec} and Andrea {Němcová} and Ivana {Márová}",
  title="Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth",
  annote="Carotenoids are one of the most abundant natural pigment and they have great potential for wide industrial utilization in food and feed industry, cosmetics, pharmacy and even electronics.Carotenoids can be obtained by means of biotechnological production using so-called red yeasts strains while utilizing waste substrates.
Red yeast Cystofilobasidium capitatum CCY 10-1-2 autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells.Yeasts were cultivated in 4L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and RP-HPLC/PDA determination of pigments. To compare FLIM images and coupled data (obtained by CLSM) with model systems, FLIM (fluorescence lifetime imaging) analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, an with constant addition of beta- carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too.
Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoids:ergosterol clusters in different compartments of yeast cell. For more information about pigment forms, their changes during yeast growth and potential physiological significance, additional investigations should be performed
",
  address="Institute of Chemistry, Slovak Academy of Sciences, Bratislava",
  booktitle="43rd Annual Conference on Yeasts - Book of Abstracts",
  chapter="133224",
  edition="1",
  howpublished="print",
  institution="Institute of Chemistry, Slovak Academy of Sciences, Bratislava",
  year="2016",
  month="may",
  pages="54--54",
  publisher="Institute of Chemistry, Slovak Academy of Sciences, Bratislava",
  type="abstract"
}