Publication detail

Multimode fibre: Light-sheet microscopy at the tip of a needle

PLÖSCHNER, M. KOLLÁROVÁ, V. DOSTÁL, Z. NYLK, J. BARTON-OWEN, T. FERRIER, D. CHMELÍK, R. DHOLAKIA, K. ČIŽMÁR, T.

Original Title

Multimode fibre: Light-sheet microscopy at the tip of a needle

English Title

Multimode fibre: Light-sheet microscopy at the tip of a needle

Type

journal article in Web of Science

Language

en

Original Abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

English abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Keywords

Light-sheet fluorescence microscopy; digital holography

RIV year

2015

Released

14.12.2015

Publisher

Springer Nature

Pages from

1

Pages to

7

Pages count

7

URL

Full text in the Digital Library

BibTex


@article{BUT119568,
  author="Martin {Plöschner} and Věra {Kollárová} and Zbyněk {Dostál} and Jonathan {Nylk} and Thomas {Barton-Owen} and David E.K. {Ferrier} and Radim {Chmelík} and Kishan {Dholakia} and Tomáš {Čižmár}",
  title="Multimode fibre: Light-sheet microscopy at the tip of a needle",
  annote="Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in
the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our
new technique, based on digital holography, enables delivery of the light-sheet through a multimode
optical fibre – an optical element with extremely small footprint, yet permitting complex control of light
transport processes within. We show that this approach supports some of the most advanced methods
in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate
the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane
illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose
gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating
operculum prongs of Spirobranchus lamarcki.",
  address="Springer Nature",
  chapter="119568",
  doi="10.1038/srep18050",
  howpublished="online",
  institution="Springer Nature",
  number="1",
  volume="5",
  year="2015",
  month="december",
  pages="1--7",
  publisher="Springer Nature",
  type="journal article in Web of Science"
}