Publication detail

Automated alignment method for coherence-controlled holographic microscope

DOSTÁL, Z. SLABÝ, T. KVASNICA, L. LOŠŤÁK, M. KŘÍŽOVÁ, A. CHMELÍK, R.

Original Title

Automated alignment method for coherence-controlled holographic microscope

English Title

Automated alignment method for coherence-controlled holographic microscope

Type

journal article in Web of Science

Language

en

Original Abstract

Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.

English abstract

Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.

Keywords

holographic microscopy; quantitative phase imaging; automated alignment; holographic signal

RIV year

2015

Released

28.10.2015

Publisher

SPIE

ISBN

1083-3668

Periodical

JOURNAL OF BIOMEDICAL OPTICS

Year of study

20

Number

11

State

US

Pages from

1

Pages to

8

Pages count

8

URL

Full text in the Digital Library

Documents

BibTex


@article{BUT117913,
  author="Zbyněk {Dostál} and Tomáš {Slabý} and Lukáš {Kvasnica} and Martin {Lošťák} and Aneta {Křížová} and Radim {Chmelík}",
  title="Automated alignment method for coherence-controlled holographic microscope",
  annote="Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative
phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.",
  address="SPIE",
  chapter="117913",
  doi="10.1117/1.JBO.20.11.111215",
  howpublished="online",
  institution="SPIE",
  number="11",
  volume="20",
  year="2015",
  month="october",
  pages="1--8",
  publisher="SPIE",
  type="journal article in Web of Science"
}