Publication detail
Automated alignment method for coherence-controlled holographic microscope
DOSTÁL, Z. SLABÝ, T. KVASNICA, L. LOŠŤÁK, M. KŘÍŽOVÁ, A. CHMELÍK, R.
Original Title
Automated alignment method for coherence-controlled holographic microscope
English Title
Automated alignment method for coherence-controlled holographic microscope
Type
journal article in Web of Science
Language
en
Original Abstract
Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.
English abstract
Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.
Keywords
holographic microscopy; quantitative phase imaging; automated alignment; holographic signal
RIV year
2015
Released
28.10.2015
Publisher
SPIE
ISBN
1083-3668
Periodical
JOURNAL OF BIOMEDICAL OPTICS
Year of study
20
Number
11
State
US
Pages from
1
Pages to
8
Pages count
8
URL
Full text in the Digital Library
Documents
BibTex
@article{BUT117913,
author="Zbyněk {Dostál} and Tomáš {Slabý} and Lukáš {Kvasnica} and Martin {Lošťák} and Aneta {Křížová} and Radim {Chmelík}",
title="Automated alignment method for coherence-controlled holographic microscope",
annote="Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative
phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.",
address="SPIE",
chapter="117913",
doi="10.1117/1.JBO.20.11.111215",
howpublished="online",
institution="SPIE",
number="11",
volume="20",
year="2015",
month="october",
pages="1--8",
publisher="SPIE",
type="journal article in Web of Science"
}