Publication detail

Characterisation of cells migration through cardiac tissue using advanced microscopy techniques and Matlab simulation

BAIAZITOVA, L. SKOPALÍK, J. ČMIEL, V. CHMELÍK, J. SVOBODA, O. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Original Title

Characterisation of cells migration through cardiac tissue using advanced microscopy techniques and Matlab simulation

English Title

Characterisation of cells migration through cardiac tissue using advanced microscopy techniques and Matlab simulation

Type

conference paper

Language

en

Original Abstract

In this work chemotaxis movement of mesenchymal stromal cells (MSC) through collagen tunnel between two chambers was studied. Monitoring of migrating cells was performed on the confocal laser scanning microscope Leica TCS SP8 X equipped with the picosecond White Light Laser (WLL). Neutrophils and MSC were stained by fluorescence nanoparticles, cardiomyocytes were stained by calcein, and thus separate monitoring of migrating cells was implemented. These results were adopted into the mathematical diffusion model created in Matlab software.

English abstract

In this work chemotaxis movement of mesenchymal stromal cells (MSC) through collagen tunnel between two chambers was studied. Monitoring of migrating cells was performed on the confocal laser scanning microscope Leica TCS SP8 X equipped with the picosecond White Light Laser (WLL). Neutrophils and MSC were stained by fluorescence nanoparticles, cardiomyocytes were stained by calcein, and thus separate monitoring of migrating cells was implemented. These results were adopted into the mathematical diffusion model created in Matlab software.

Keywords

Mesenchymal stromal cells, neutrophils, myocytes, nanoparticles, calcein, migrating cells, confocal microscopy,

RIV year

2015

Released

01.09.2015

Publisher

Computing in Cardiology 2015

Location

Nice, France

ISBN

978-1-5090-0684-7

Book

Computing in Cardiology 2015

Edition number

42

Pages from

1125

Pages to

1128

Pages count

4

BibTex


@inproceedings{BUT115173,
  author="Larisa {Baiazitova} and Josef {Skopalík} and Vratislav {Čmiel} and Jiří {Chmelík} and Ondřej {Svoboda} and Ivo {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="Characterisation of cells migration through cardiac tissue using advanced microscopy techniques and Matlab simulation",
  annote="In this work chemotaxis movement of mesenchymal stromal cells (MSC) through collagen tunnel between two chambers was studied. Monitoring of migrating cells was performed on the confocal laser scanning microscope Leica TCS SP8 X equipped with the picosecond White Light Laser (WLL). Neutrophils and MSC were stained by fluorescence nanoparticles, cardiomyocytes were stained by calcein, and thus separate monitoring of migrating cells was implemented. These results were adopted into the mathematical diffusion model created in Matlab software.",
  address="Computing in Cardiology 2015",
  booktitle="Computing in Cardiology 2015",
  chapter="115173",
  doi="10.1109/CIC.2015.7411113",
  howpublished="online",
  institution="Computing in Cardiology 2015",
  year="2015",
  month="september",
  pages="1125--1128",
  publisher="Computing in Cardiology 2015",
  type="conference paper"
}