Publication detail

Quantitative phase imaging through scattering media

KOLLÁROVÁ, V. ČOLLÁKOVÁ, J. DOSTÁL, Z. SLABÝ, T. VESELÝ, P. CHMELÍK, R.

Original Title

Quantitative phase imaging through scattering media

English Title

Quantitative phase imaging through scattering media

Type

conference paper

Language

en

Original Abstract

Coherence-controlled holographic microscope (CCHM) is an off-axis holographic system. It enables observation of a sample and its quantitative phase imaging with coherent as well as with incoherent illumination. The spatial and temporal coherence can be modified and thus also the quality and type of the image information. The coherent illumination provides numerical refocusing in wide depth range similarly to a classic coherent-light digital holographic microscopy (HM). Incoherent-light HM is characterized by a high quality, coherence-noise-free imaging with up to twice higher resolution compared to coherent illumination. Owing to an independent, free of sample reference arm of the CCHM the low spatial light coherence induces coherence-gating effect. This makes possible to observe specimen also through scattering media. We have described theoretically and simulated numerically imaging of a two dimensional object through a scattering layer by CCHM using the linear systems theory. We have investigated both strongly and weakly scattering media characterized by different amount of ballistic and diffuse light. The influence of a scattering layer on the quality of a phase signal is discussed for both types of the scattering media. A strong dependence of the imaging process on the light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with model samples, as well as real biologic objects particularly then by time-lapse observations of live cells reactions to substances producing optically turbid emulsion.

English abstract

Coherence-controlled holographic microscope (CCHM) is an off-axis holographic system. It enables observation of a sample and its quantitative phase imaging with coherent as well as with incoherent illumination. The spatial and temporal coherence can be modified and thus also the quality and type of the image information. The coherent illumination provides numerical refocusing in wide depth range similarly to a classic coherent-light digital holographic microscopy (HM). Incoherent-light HM is characterized by a high quality, coherence-noise-free imaging with up to twice higher resolution compared to coherent illumination. Owing to an independent, free of sample reference arm of the CCHM the low spatial light coherence induces coherence-gating effect. This makes possible to observe specimen also through scattering media. We have described theoretically and simulated numerically imaging of a two dimensional object through a scattering layer by CCHM using the linear systems theory. We have investigated both strongly and weakly scattering media characterized by different amount of ballistic and diffuse light. The influence of a scattering layer on the quality of a phase signal is discussed for both types of the scattering media. A strong dependence of the imaging process on the light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with model samples, as well as real biologic objects particularly then by time-lapse observations of live cells reactions to substances producing optically turbid emulsion.

Keywords

Digital holograhy, Microscopy, Imaging through scattering media, Imaging through turbid media, Coherencecontrolled holographic microscopy, Quantitative phase imaging

RIV year

2015

Released

11.03.2015

Publisher

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

Location

San Francisco, CA

ISBN

978-1-62841-426-4

Book

Quantitative Phase Imaging

Pages from

93360T-1

Pages to

93360T-8

Pages count

8

URL

BibTex


@inproceedings{BUT115035,
  author="Věra {Kollárová} and Jana {Čolláková} and Zbyněk {Dostál} and Tomáš {Slabý} and Pavel {Veselý} and Radim {Chmelík}",
  title="Quantitative phase imaging through scattering media",
  annote="Coherence-controlled holographic microscope (CCHM) is an off-axis holographic system. It enables observation of a sample and its quantitative phase imaging with coherent as well as with incoherent illumination. The spatial and temporal coherence can be modified and thus also the quality and type of the image information. The coherent illumination provides numerical refocusing in wide depth range similarly to a classic coherent-light digital holographic microscopy (HM).  Incoherent-light HM is characterized by a high quality, coherence-noise-free imaging with up to twice higher resolution compared to coherent illumination. Owing to an independent, free of sample reference arm of the CCHM the low spatial light coherence induces coherence-gating effect. This makes possible to observe specimen also through scattering media.
We have described theoretically and simulated numerically imaging of a two dimensional object through a scattering layer by CCHM using the linear systems theory. We have investigated both strongly and weakly scattering media characterized by different amount of ballistic and diffuse light. The influence of a scattering layer on the quality of a phase signal is discussed for both types of the scattering media. A strong dependence of the imaging process on the light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with model samples, as well as real biologic objects particularly then by time-lapse observations of live cells reactions to substances producing optically turbid emulsion.",
  address="SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA",
  booktitle="Quantitative Phase Imaging",
  chapter="115035",
  doi="10.1117/12.2078003",
  howpublished="print",
  institution="SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA",
  year="2015",
  month="march",
  pages="93360T-1--93360T-8",
  publisher="SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA",
  type="conference paper"
}