Publication detail

Hyaluronan-cetyltrimethylammonium bromide interactions in aqueous and physiological solution studied by fluorescence techniques

HOLÍNKOVÁ, P. MRAVEC, F. PEKAŘ, M.

Original Title

Hyaluronan-cetyltrimethylammonium bromide interactions in aqueous and physiological solution studied by fluorescence techniques

English Title

Hyaluronan-cetyltrimethylammonium bromide interactions in aqueous and physiological solution studied by fluorescence techniques

Type

conference paper

Language

en

Original Abstract

The aim of this work was to study systems composed of negatively charged polyelectrolyte – hyaluronan and positively charged surfactant – cetyltrimethylammonium bromide (CTAB), which could be potential used for targeted drug delivery. Two concentration series was mixed – in an aqueous solution and in 0.15 mol l-1 sodium chloride (simply physiological solution). In the series, the concentration of hyaluronan was constant and the concentration of CTAB (well below the critical micelle concentration in water solution) was increased. The interactions between these components were studied in terms of the formation of hydrophobic domains, which were important for solubilization of molecules of hydrophobic drugs. The intensity of fluorescence, steady-state anisotropy and lifetime of the samples were measured. Perylene was chosen as fluorescent probe due to its hydrophobicity. It was found that in aqueous solution, small hydrophobic domains linked to hyaluronan chains are formed. Then an increasing concentration of CTAB leads to phase separation and formation of the condensed phase. Addition of sodium chloride to the samples leads to the reorganization of this system – the condensed phase is not created and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.

English abstract

The aim of this work was to study systems composed of negatively charged polyelectrolyte – hyaluronan and positively charged surfactant – cetyltrimethylammonium bromide (CTAB), which could be potential used for targeted drug delivery. Two concentration series was mixed – in an aqueous solution and in 0.15 mol l-1 sodium chloride (simply physiological solution). In the series, the concentration of hyaluronan was constant and the concentration of CTAB (well below the critical micelle concentration in water solution) was increased. The interactions between these components were studied in terms of the formation of hydrophobic domains, which were important for solubilization of molecules of hydrophobic drugs. The intensity of fluorescence, steady-state anisotropy and lifetime of the samples were measured. Perylene was chosen as fluorescent probe due to its hydrophobicity. It was found that in aqueous solution, small hydrophobic domains linked to hyaluronan chains are formed. Then an increasing concentration of CTAB leads to phase separation and formation of the condensed phase. Addition of sodium chloride to the samples leads to the reorganization of this system – the condensed phase is not created and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.

Keywords

Fluorescence anisotropy, interaction, perylene, minimicelles, hydrophobic domains, CAC, critical aggregation concentration

RIV year

2014

Released

04.12.2014

Location

Brno

ISBN

978-80-214-5078-3

Book

Chemie je život 2014 - Sborník příspěvků

Edition

1

Edition number

1

Pages from

229

Pages to

234

Pages count

6

BibTex


@inproceedings{BUT113908,
  author="Petra {Holínková} and Filip {Mravec} and Miloslav {Pekař}",
  title="Hyaluronan-cetyltrimethylammonium bromide interactions in aqueous and physiological solution studied by fluorescence techniques",
  annote="The aim of this work was to study systems composed of negatively charged polyelectrolyte – hyaluronan and positively charged surfactant – cetyltrimethylammonium bromide (CTAB), which could be potential used for targeted drug delivery. Two concentration series was mixed – in an aqueous solution and in 0.15 mol l-1 sodium chloride (simply physiological solution). In the series, the concentration of hyaluronan was constant and the concentration of CTAB (well below the critical micelle concentration in water solution) was increased. The interactions between these components were studied in terms of the formation of hydrophobic domains, which were important for solubilization of molecules of hydrophobic drugs. The intensity of fluorescence, steady-state anisotropy and lifetime of the samples were measured. Perylene was chosen as fluorescent probe due to its hydrophobicity. It was found that in aqueous solution, small hydrophobic domains linked to hyaluronan chains are formed.  Then an increasing concentration of CTAB leads to phase separation and formation of the condensed phase. Addition of sodium chloride to the samples leads to the reorganization of this system – the condensed phase is not created and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.",
  booktitle="Chemie je život 2014 - Sborník příspěvků",
  chapter="113908",
  edition="1",
  howpublished="electronic, physical medium",
  year="2014",
  month="december",
  pages="229--234",
  type="conference paper"
}