Publication detail

Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene

NGUYEN, H. TMEJOVÁ, K. KREJČOVÁ, L. HYNEK, D. KOPEL, P. KYNICKÝ, J. ADAM, V. KIZEK, R.

Original Title

Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene

Type

journal article in Web of Science

Language

English

Original Abstract

This paper comes with the application of the Brdicka method for PNA testing and characterization. The classical approach based on the catalytic electroreduction of protons was used. In this study electrochemical determination of PNA in Brdicka solution was investigated by two techniques of differential pulse voltammetry (DPV) and DPV coupled with adsorptive stripping technique. The instrumentation of experiment was improved by manufacturing new holder for cooling electrochemical cell necessary for measurements in temperature sensitive electrolyte. This holder was made using 3D printing system from polylactide (PLA), biodegradable thermoplastic aliphatic polyester. AdT DPV produced four typical peaks (Co1 peak at potential -1.00 + - 0.05 V, RS2CO at potential -1.25 + - 0.05 V, Cat1 at potential -1.35 + - 0.05 V and Cat2 at potential -1.55 + - 0.05 V). Cat2 peak height was strongly depended on accumulation time, temperature of electrolyte, and concentration of PNA

Keywords

PNA; Brdicka Electrolyte; 3D printing; Voltammetry; Adsorptive Transfer Technique

Authors

NGUYEN, H.; TMEJOVÁ, K.; KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; KYNICKÝ, J.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 7. 2014

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

9

Number

7

State

Republic of Serbia

Pages from

3364

Pages to

3373

Pages count

10

BibTex

@article{BUT109362,
  author="Hoai Viet {Nguyen} and Kateřina {Tmejová} and Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Jindřich {Kynický} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3364--3373",
  issn="1452-3981"
}