Publication detail

The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

MICHALICOVÁ, P. BORŮVKOVÁ, T. MRAVEC, F. ČÁSLAVSKÝ, J. PEKAŘ, M.

Original Title

The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

English Title

The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

Type

abstract

Language

en

Original Abstract

Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.

English abstract

Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.

Keywords

hyaluronan, drying, tert-butanol, gas chromatography

Released

07.09.2014

Publisher

Czech Chemical Society

Pages from

772

Pages to

772

Pages count

1

BibTex


@misc{BUT109297,
  author="Petra {Michalicová} and Tereza {Borůvková} and Filip {Mravec} and Josef {Čáslavský} and Miloslav {Pekař}",
  title="The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes",
  annote="Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.",
  address="Czech Chemical Society",
  booktitle="Chemické listy",
  chapter="109297",
  howpublished="print",
  institution="Czech Chemical Society",
  number="8",
  year="2014",
  month="september",
  pages="772--772",
  publisher="Czech Chemical Society",
  type="abstract"
}