Publication detail

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

BĚLKA, M. LIPPAY, J. LÍZAL, F. JEDELSKÝ, J. JÍCHA, M.

Original Title

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

English Title

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

Type

conference paper

Language

en

Original Abstract

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

English abstract

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

Keywords

Aerosol deposition, optical microscopy, fluorescent spectroscopy, human lungs

RIV year

2013

Released

25.03.2014

Publisher

E D P SCIENCES

Location

FRANCE

ISBN

978-80-260-5375-0

Book

EPJ Web of Conferences

Pages from

82

Pages to

85

Pages count

4

BibTex


@inproceedings{BUT104093,
  author="Miloslav {Bělka} and Josef {Lippay} and František {Lízal} and Jan {Jedelský} and Miroslav {Jícha}",
  title="Comparison of methods for evaluation of aerosol deposition in the model of human lungs",
  annote="It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.",
  address="E D P SCIENCES",
  booktitle="EPJ Web of Conferences",
  chapter="104093",
  doi="10.1051/epjconf/20146702006",
  howpublished="online",
  institution="E D P SCIENCES",
  number="2014",
  year="2014",
  month="march",
  pages="82--85",
  publisher="E D P SCIENCES",
  type="conference paper"
}