Publication detail

DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices

CHROBOKOVÁ, M. RITTICH, B. ŠPANOVÁ, A. HORÁK, D.

Original Title

DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices

Type

conference paper

Language

English

Original Abstract

Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.

Keywords

magnetic particles, DNA, microorganisms, foods; polymerase chain reaction

Authors

CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.

RIV year

2013

Released

21. 3. 2013

Publisher

Československá spoločnosť mikrobiologická

Location

Bratislava-Praha

ISBN

978-80-971422-0-9

Book

Čo nového v mikrobiologii

Pages from

94

Pages to

98

Pages count

222

BibTex

@inproceedings{BUT99830,
  author="Maria {Chroboková} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák}",
  title="DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices",
  booktitle="Čo nového v mikrobiologii",
  year="2013",
  pages="94--98",
  publisher="Československá spoločnosť mikrobiologická",
  address="Bratislava-Praha",
  isbn="978-80-971422-0-9"
}