Publication detail

Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure

ŠIMŠÍKOVÁ, M. ANTALÍK, M. KAŇUCHOVÁ, M. ŠKVARLA, J.

Original Title

Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure

Type

journal article - other

Language

English

Original Abstract

Nanoparticle–protein conjugates have potential for numerous applications due to the combination of theproperties of both components. In this paper we studied the conjugation of horse heart cytochrome c withZnO nanoparticles modified by mercaptoacetic acid (MAA) which may be a material with great potentialin anticancer therapy as a consequence of synergic effect of both components. Cyt c adsorption to theZnO–MAA NPs surface was studied by UV–vis spectroscopy and by a dynamic light scattering in variouspH. The results indicate that the optimal pH for the association of protein with modified nanoparticles isin range 5.8–8.5 where 90–96% of cytochrome c was assembled on ZnO–MAA nanoparticles.The interaction of proteins with nanoparticles often results in denaturation or loss of protein func-tion. Our observations from UV–vis spectroscopy and circular dichroism performed preserved proteinstructure after the interaction with modified nanoparticles

Keywords

ZnO nanoparticles; Mercaptoacetic acid; Cytochrome c; Protein interaction

Authors

ŠIMŠÍKOVÁ, M.; ANTALÍK, M.; KAŇUCHOVÁ, M.; ŠKVARLA, J.

RIV year

2013

Released

1. 8. 2013

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

59

Number

1

State

Kingdom of the Netherlands

Pages from

235

Pages to

241

Pages count

7

BibTex

@article{BUT99659,
  author="Michaela {Šimšíková} and Marián {Antalík} and Mária {Kaňuchová} and Jiří {Škvarla}",
  title="Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2013",
  volume="59",
  number="1",
  pages="235--241",
  issn="0141-8130"
}