Publication detail

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

BABULA, P. VODIČKA, O. ADAM, V. KUMMEROVÁ, M. HAVEL, L. HOŠEK, J. PROVAZNÍK, I. ŠKUTKOVÁ, H. BEKLOVÁ, M. KIZEK, R.

Original Title

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

Czech Title

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

English Title

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

Type

journal article

Language

en

Original Abstract

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000uM, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.

Czech abstract

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000uM, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.

English abstract

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000uM, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.

Keywords

Fluoranthene, BY-2 cells, Viability, Programmed cell death, Fluoranthene accumulation

RIV year

2012

Released

21.01.2012

Pages from

117

Pages to

126

Pages count

14

BibTex


@article{BUT88856,
  author="Petr {Babula} and Ondřej {Vodička} and Vojtěch {Adam} and Marie {Kummerová} and Ladislav {Havel} and Jan {Hošek} and Ivo {Provazník} and Helena {Škutková} and Miroslava {Beklová} and René {Kizek}",
  title="Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture",
  annote="Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the
living environment, which are present in air, soils, freshwater, seawater and sediments. They have very
substantial effects on all living organisms including plants and animals. Plants represent important point
in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on
plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant
cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to
the fluoranthene in concentration range from 0 to 1000uM, duration of treatment was 120 h, respectively
5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led
to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in
lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which
resulted in damage of biomembranes and disruption of their semipermeability and initiation of process
of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene
and mechanism of its action.",
  chapter="88856",
  number="78",
  volume="2012",
  year="2012",
  month="january",
  pages="117--126",
  type="journal article"
}