Publication detail

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

RITTICH, B. ŠPANOVÁ, A. TRACHTOVÁ, Š. HORÁK, D.

Original Title

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

Type

abstract

Language

English

Original Abstract

Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.

Keywords

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR

Authors

RITTICH, B.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.

Released

30. 6. 2010

Publisher

VUTIUM

Location

Brno

ISBN

978-80-214-4112-5

Book

Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.

Pages from

151

Pages to

151

Pages count

1

BibTex

@misc{BUT72665,
  author="RITTICH, B. and ŠPANOVÁ, A. and TRACHTOVÁ, Š. and HORÁK, D.",
  title="Magnetic Microspheres for DNA Diagnostics and DNA Delivery",
  booktitle="Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.",
  year="2010",
  pages="151--151",
  publisher="VUTIUM",
  address="Brno",
  isbn="978-80-214-4112-5",
  note="abstract"
}