Publication detail

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Zuzana Grosmanova, Jan Krejci, Jaroslav Tynek, Petr Cuhra a Sona Barsova

Original Title

Comparison of biosensoric and chromatographic methods for the detection of pesticides

English Title

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Type

journal article

Language

en

Original Abstract

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is solved by the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor match those of chromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after measurements using the biosensor.

English abstract

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is solved by the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor match those of chromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after measurements using the biosensor.

Keywords

Acetylcholine esterase; Food analysis; Enzyme-based biosensor

RIV year

2005

Released

15.10.2005

Publisher

Taylor & Francis

Pages from

885

Pages to

892

Pages count

8

BibTex


@article{BUT45537,
  author="Zuzana {Grosmanová} and Jan {Krejčí} and Jaroslav {Týnek}",
  title="Comparison of biosensoric and chromatographic methods for the detection of pesticides",
  annote="Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for
measuring fruit and vegetable samples that had first been analysed using gas and highperformance
liquid chromatography. The output signal for the biosensors is the current, which
is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the
inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE
biosensors are not easily reproducible. This problem is solved by the choice of an arbitrary
toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the
relative inhibition of the sample against the relative inhibition of Syntostigmin. Results
obtained from the biosensor match those of chromatography in 19 out of 38 total
measurements made and for nine out of 19 positive samples. The confirmation rate was
50%. Future work must check the limit of 0.1 and the independent control of inhibiting
pesticides contents after measurements using the biosensor.",
  address="Taylor & Francis",
  chapter="45537",
  institution="Taylor & Francis",
  journal="International Journal of Environmental Analytical Chemistry",
  number="12-13",
  volume="85",
  year="2005",
  month="october",
  pages="885",
  publisher="Taylor & Francis",
  type="journal article"
}