Publication detail

Directionality of developing skeletal muscles is set by mechanical forces

Sunadome, K. Erickson, A.G. Kah, D. Fabry, B. Adori, C. Kameneva, P. Faure, L. Kanatani, S. Kaucka, M. Ellström, I.D. Tesarova, M. Zikmund, T. Kaiser, J. Edwards, S. Maki, K. Adachi, T. Yamamoto, T. Fried K. Adameyko, I.

Original Title

Directionality of developing skeletal muscles is set by mechanical forces

Type

journal article in Web of Science

Language

English

Original Abstract

The mechanisms that drive myocyte orientation and fusion to control muscle directionality are not well understood. Here authors show that the developing skeleton produces mechanical tension that instructs the directional outgrowth of skeletal muscles. Formation of oriented myofibrils is a key event in musculoskeletal development. However, the mechanisms that drive myocyte orientation and fusion to control muscle directionality in adults remain enigmatic. Here, we demonstrate that the developing skeleton instructs the directional outgrowth of skeletal muscle and other soft tissues during limb and facial morphogenesis in zebrafish and mouse. Time-lapse live imaging reveals that during early craniofacial development, myoblasts condense into round clusters corresponding to future muscle groups. These clusters undergo oriented stretch and alignment during embryonic growth. Genetic perturbation of cartilage patterning or size disrupts the directionality and number of myofibrils in vivo. Laser ablation of musculoskeletal attachment points reveals tension imposed by cartilage expansion on the forming myofibers. Application of continuous tension using artificial attachment points, or stretchable membrane substrates, is sufficient to drive polarization of myocyte populations in vitro. Overall, this work outlines a biomechanical guidance mechanism that is potentially useful for engineering functional skeletal muscle.

Keywords

muscles, myocyte, morphogenesis, cartilage development, cell polarity, skeletal muscle

Authors

Sunadome, K.; Erickson, A.G.; Kah, D.; Fabry, B.; Adori, C.; Kameneva, P.; Faure, L.; Kanatani, S.; Kaucka, M.; Ellström, I.D.; Tesarova, M.; Zikmund, T.; Kaiser, J.; Edwards, S.; Maki, K.; Adachi, T.; Yamamoto, T.; Fried K.; Adameyko, I.

Released

27. 5. 2023

Publisher

NATURE PORTFOLIO

Location

BERLIN

ISBN

2041-1723

Periodical

NATURE COMMUNICATIONS

Year of study

14

Number

3060

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

24

Pages count

24

URL

https://www.nature.com/articles/s41467-023-38647-7

BibTex

@article{BUT185233,
  author="Sunadome, K. and Erickson, A.G. and Kah, D. and Fabry, B. and Adori, C. and Kameneva, P. and Faure, L. and Kanatani, S. and Kaucka, M. and Ellström, I.D. and Tesarova, M. and Zikmund, T. and Kaiser, J. and Edwards, S. and Maki, K. and Adachi, T. and Yamamoto, T. and Fried K. and Adameyko, I.",
  title="Directionality of developing skeletal muscles is set by mechanical forces",
  journal="NATURE COMMUNICATIONS",
  year="2023",
  volume="14",
  number="3060",
  pages="1--24",
  doi="10.1038/s41467-023-38647-7",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-023-38647-7"
}