Publication detail

Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells

MRÁZOVÁ, K. BAČOVSKÝ, J. ŠEDRLOVÁ, Z. SLANINOVÁ, E. OBRUČA, S. FRITZ, I. KRYŽÁNEK, V.

Original Title

Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells

Type

journal article in Web of Science

Language

English

Original Abstract

Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.

Keywords

low voltage electron microscopy; uranyl acetate; contrasting agents; transmission electron microscopy; Synechocystis; polyhydroxyalkanoates

Authors

MRÁZOVÁ, K.; BAČOVSKÝ, J.; ŠEDRLOVÁ, Z.; SLANINOVÁ, E.; OBRUČA, S.; FRITZ, I.; KRYŽÁNEK, V.

Released

29. 3. 2023

Publisher

MDPI

ISBN

2076-2607

Periodical

Microorganisms

Year of study

11

Number

4

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/2076-2607/11/4/888

Full text in the Digital Library

http://hdl.handle.net/11012/213621

BibTex

@article{BUT184203,
  author="Kateřina {Mrázová} and Jaromír {Bačovský} and Zuzana {Šedrlová} and Eva {Slaninová} and Stanislav {Obruča} and Ines {Fritz} and Vladislav {Krzyžánek}",
  title="Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells",
  journal="Microorganisms",
  year="2023",
  volume="11",
  number="4",
  pages="1--13",
  doi="10.3390/microorganisms11040888",
  issn="2076-2607",
  url="https://www.mdpi.com/2076-2607/11/4/888"
}