Publication detail

Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology

ŠTĚPÁNKOVÁ, H. MICHÁLKOVÁ, H. ŠPLÍCHAL, Z. RICHTERA, L. ŠVEC, P. VACULOVIČ, T. PŘIBYL, J. KORMUNDA, M. REX, S. ADAM, V. HEGER, Z.

Original Title

Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology

Type

journal article in Web of Science

Language

English

Original Abstract

Although the general concept of nanotechnology relies on exploitation of size-dependent properties of nano -scaled materials, the relation between the size/morphology of nanoparticles with their biological activity re-mains not well understood. Therefore, we aimed at investigating the biological activity of Se nanoparticles, one of the most promising candidates of nanomaterials for biomedicine, possessing the same crystal structure, but differing in morphology (nanorods vs. spherical particles) and aspect ratios (AR, 11.5 vs. 22.3 vs. 1.0) in human cells and BALB/c mice. Herein, we report that in case of nanorod-shaped Se nanomaterials, AR is a critical factor describing their cytotoxicity and biocompatibility. However, spherical nanoparticles (AR 1.0) do not fit this statement and exhibit markedly higher cytotoxicity than lower-AR Se nanorods. Beside of cytotoxicity, we also show that morphology and size substantially affect the uptake and intracellular fate of Se nanomaterials. In line with in vitro data, in vivo i.v. administration of Se nanomaterials revealed the highest toxicity for higher-AR nanorods followed by spherical nanoparticles and lower-AR nanorods. Moreover, we revealed that Se nano -materials are able to alter intracellular redox homeostasis, and affect the acidic intracellular vesicles and cyto-skeletal architecture in a size-and morphology-dependent manner. Although the tested nanoparticles were produced from the similar sources, their behavior differs markedly, since each type is promising for several various application scenarios, and the presented testing protocol could serve as a concept standardizing the biological relevance of the size and morphology of the various types of nanomaterials and nanoparticles.

Keywords

Aspect ratio; Biocompatibility; Nanotoxicology; Nanorods

Authors

ŠTĚPÁNKOVÁ, H.; MICHÁLKOVÁ, H.; ŠPLÍCHAL, Z.; RICHTERA, L.; ŠVEC, P.; VACULOVIČ, T.; PŘIBYL, J.; KORMUNDA, M.; REX, S.; ADAM, V.; HEGER, Z.

Released

1. 2. 2023

ISBN

2452-199X

Periodical

Bioactive Materials

Year of study

20

Number

1

State

People's Republic of China

Pages from

489

Pages to

500

Pages count

12

URL

https://doi.org/10.1016/j.bioactmat.2022.06.014

Full text in the Digital Library

http://hdl.handle.net/11012/209249

BibTex

@article{BUT183017,
  author="Hana {Štěpánková} and Hana {Michálková} and Zbyněk {Šplíchal} and Lukáš {Richtera} and Pavel {Švec} and Tomáš {Vaculovič} and Jan {Přibyl} and Martin {Kormunda} and Simona {Rex} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology",
  journal="Bioactive Materials",
  year="2023",
  volume="20",
  number="1",
  pages="489--500",
  doi="10.1016/j.bioactmat.2022.06.014",
  issn="2452-199X",
  url="https://doi.org/10.1016/j.bioactmat.2022.06.014"
}