Publication detail

The effect of aminooxy-linkers' structure on the mechanical properties of hyaluronan-oxime hydrogels

ŠEDOVÁ, P., BUFFA, R., KOČÍ, T., KOVÁŘOVÁ, L., BEDNAŘÍK, J., VÁGNEROVÁ, H., VELEBNÝ V.

Original Title

The effect of aminooxy-linkers' structure on the mechanical properties of hyaluronan-oxime hydrogels

Type

journal article in Web of Science

Language

English

Original Abstract

The wide range of hydrogel applications is a result of the optimization of gelation. Herein, the various aminooxylinkers were synthesized to study the kinetics of gelation and mechanical properties of hydrogels prepared by crosslinking of aldehyde-modified N-acetylglucosamine (GlcNAc) of hyaluronan (Delta HA-CHO: 4,5-anhydro-6-(GlcNAc)-oxo hyaluronan or HA-CHO: 6-(GlcNAc)-oxo hyaluronan). Structural characteristics of the linkers (length, number of functional groups and rigidity) and the polymer (effect of the -C=C- double bond) were investigated in detail and showed decreasing mechanical stiffness with increasing hydrophobic character of linker and effect of its rigidity/flexibility on imine conversion. Besides known linear bis(aminooxy)alkanes with different lengths of the chain, new multifunctional aminooxy-linkers: 1,3,5tris(aminooxymethyl)benzene and multikis(6-aminooxy-6-deoxy)-beta-cyclodextrin were prepared and shortened the gelation time. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) analysis showed that despite the aminooxylinkers ' cytotoxicity at higher concentrations, the final oxime hydrogels are non-cytotoxic and therefore are suitable for medicinal applications.

Keywords

polymer gels; hyaluronan; rheology; oxyamine

Authors

ŠEDOVÁ, P., BUFFA, R., KOČÍ, T., KOVÁŘOVÁ, L., BEDNAŘÍK, J., VÁGNEROVÁ, H., VELEBNÝ V.

Released

17. 2. 2022

Publisher

BUDAPEST UNIV TECHNOL & ECON

Location

BUDAPEST

ISBN

1788-618X

Periodical

EXPRESS POLYM LETT

Year of study

16

Number

3

State

Hungary

Pages from

265

Pages to

278

Pages count

14

URL

https://web-p-ebscohost-com.ezproxy.lib.vutbr.cz/ehost/pdfviewer/pdfviewer?vid=0&sid=8dd8c0ea-6e22-44c5-b745-c30352736dd8%40redis

BibTex

@article{BUT182859,
  author="Lenka {Kovářová}",
  title="The effect of aminooxy-linkers' structure on the mechanical properties of hyaluronan-oxime hydrogels",
  journal="EXPRESS POLYM LETT",
  year="2022",
  volume="16",
  number="3",
  pages="265--278",
  doi="10.3144/expresspolymlett.2022.21",
  issn="1788-618X",
  url="https://web-p-ebscohost-com.ezproxy.lib.vutbr.cz/ehost/pdfviewer/pdfviewer?vid=0&sid=8dd8c0ea-6e22-44c5-b745-c30352736dd8%40redis"
}