Publication detail

Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

Jaros, J. Petrov, M.Tesarova, M. Hampl, A.

Original Title

Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

Type

journal article in Scopus

Language

English

Original Abstract

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

Keywords

3D visualization; Image reconstruction; Image segmentation; Morphology; Organoid; SBF-SEM; Scanning electron microscopy; Serial block-face; Spheroid; Stem cell; Ultrastructure

Authors

Jaros, J.; Petrov, M.;Tesarova, M.; Hampl, A.

Released

1. 1. 2017

ISBN

1940-6029

Periodical

Methods in Molecular Biology

Year of study

1612

Number

1

State

United States of America

Pages from

417

Pages to

431

Pages count

15

URL

https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TITLE-ABS-KEY%28Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy%29&relpos=0&citeCnt=3&searchTerm=#funding-details

BibTex

@article{BUT176993,
  author="Jaros, J. and Petrov, M. and Tesarova, M. and Hampl, A.",
  title="Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy",
  journal="Methods in Molecular Biology",
  year="2017",
  volume="1612",
  number="1",
  pages="417--431",
  doi="10.1007/978-1-4939-7021-6\{_}30",
  issn="1940-6029",
  url="https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TITLE-ABS-KEY%28Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy%29&relpos=0&citeCnt=3&searchTerm=#funding-details"
}