Publication detail

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

ASSI, N. NEJDL, L. ZEMÁNKOVÁ, K. PAVELICOVÁ, K. BEZDĚKOVÁ, J. MACKA, M. ADAM, V. VACULOVIČOVÁ, M.

Original Title

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

Type

journal article in Web of Science

Language

English

Original Abstract

In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12-150 mu g/mL was linear with a detection limit of 3.6 mu g/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.

Keywords

thiol; molecularly imprinted polymer; polydopamine; urine

Authors

ASSI, N.; NEJDL, L.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; BEZDĚKOVÁ, J.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M.

Released

5. 7. 2021

Publisher

Springer Nature

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

11

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://doi.org/10.1038/s41598-021-93137-4

Full text in the Digital Library

http://hdl.handle.net/11012/200921

BibTex

@article{BUT172185,
  author="Navid {Assi} and Lukáš {Nejdl} and Kristýna {Zemánková} and Kristýna {Pavelicová} and Jaroslava {Bezděková} and Miroslav {Macka} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols",
  journal="Scientific Reports",
  year="2021",
  volume="11",
  number="1",
  pages="1--11",
  doi="10.1038/s41598-021-93137-4",
  issn="2045-2322",
  url="https://doi.org/10.1038/s41598-021-93137-4"
}