Publication detail

Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry

ZEMÁNKOVÁ, K. NEJDL, L. BEZDĚKOVÁ, J. VODOVÁ, M. PETERA, L. PASTOREK, A. CIVIŠ, S. KUBELÍK, P. FERUS, M. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry

Type

journal article in Web of Science

Language

English

Original Abstract

Capillary electromigration techniques have proven their capabilities in detection of variety of analytes from inorganic ions and small organic molecules through bio(macro)molecules to large analytes such as cells or nano/ micro particles. Also broad range of potential applications includes food and environmental analysis, biomedical and pharmaceutical investigations or even diagnostics. In this work, it was demonstrated that capillary micellar electrokinetic chromatography is an excellent tool extremely helpful in investigations focused on prebiotic synthesis of molecules essential for formation of life on early Earth & ndash; nucleobases. In particular, rapid separation of nucleobases (< 2 min) was achieved in 40 mM borate buffer separation electrolyte containing 60 mM sodium dodecyl sulfate as an additive. This approach enabled detection of nucleobases formed in thermolysed formamide under conditions simulating the environment occurring on early Earth. Moreover, polydopamine-based molecularly imprinted polymers specific to thymine and uracil improved detection of these low abundant products

Keywords

Capillary electrophoresis; Purine; Pyrimidine; Nucleobases; Origin of life; Molecularly imprinted polymers

Authors

ZEMÁNKOVÁ, K.; NEJDL, L.; BEZDĚKOVÁ, J.; VODOVÁ, M.; PETERA, L.; PASTOREK, A.; CIVIŠ, S.; KUBELÍK, P.; FERUS, M.; ADAM, V.; VACULOVIČOVÁ, M.

Released

1. 8. 2021

ISBN

0026-265X

Periodical

MICROCHEMICAL JOURNAL

Year of study

167

Number

106022

State

Kingdom of the Netherlands

Pages from

1

Pages to

7

Pages count

7

URL

https://doi.org/10.1016/j.microc.2021.106022

BibTex

@article{BUT171936,
  author="Kristýna {Zemánková} and Lukáš {Nejdl} and Jaroslava {Bezděková} and Milada {Vodová} and Lukáš {Petera} and Adam {Pastorek} and Svatopluk {Civiš} and Petr {Kubelík} and Martin {Ferus} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry",
  journal="MICROCHEMICAL JOURNAL",
  year="2021",
  volume="167",
  number="106022",
  pages="1--7",
  doi="10.1016/j.microc.2021.106022",
  issn="0026-265X",
  url="https://doi.org/10.1016/j.microc.2021.106022"
}