Publication detail

Structural and biological characterization of anticancer nickel(II) bis(benzimidazole) complex

MASARYK, L. TESAŘOVÁ, B. CHOQUESILLO-LAZARTE, D. MILOSAVLJEVIĆ, V. HEGER, Z. KOPEL, P.

Original Title

Structural and biological characterization of anticancer nickel(II) bis(benzimidazole) complex

Type

journal article in Web of Science

Language

English

Original Abstract

In the present study, nickel(II) complex with 2-[2-[2-(1H-benzimidazol-2-yl)ethylsulfanyl]ethyl]-1H-benzimid-azole (tebb) of formula [Ni(tebb)(2)](ClO4)(2) has been prepared and its structure was proved by X-ray crystallography. The central nickel atom is in deformed octahedral vicinity. Four nitrogen atoms of two ligands form plane of octahedral and sulfur atoms are in apical positions. Perchlorate anions are outside the coordination sphere. The coordination compound was tested for its biological activities in an array of in vitro assays. It was found that the synthesized complex possesses interesting biological activity, which is most likely related to its cell-type related uptake kinetics. The synthesized complex is readily uptaken by malignant MDA-MB-231 and CACO-2 cells with the lowest uptake by healthy Hs27 fibroblasts. The lowest IC50 values were obtained for MDA-MB-231 cells (5.2-12.7 mu M), highlighting exceptional differential cytotoxicity (IC50 values for healthy fibroblasts were 38.6-51.5 mu M). Furthermore, it was found the complex is capable to cause hydrolytic DNA cleavage, promotes an efficient DNA fragmentation and to trigger an extensive formation of intracellular reactive oxygen species. Overall, current work presents a synthesis of Ni(II) coordination compound with interesting biological behavior and with a promising potential to be further tested in pre-clinical models.

Keywords

Cytotoxicity; Biocompatibility; Nickel coordination compound; Benzimidazole; X-ray; Reactive oxygen species

Authors

MASARYK, L.; TESAŘOVÁ, B.; CHOQUESILLO-LAZARTE, D.; MILOSAVLJEVIĆ, V.; HEGER, Z.; KOPEL, P.

Released

30. 4. 2021

ISBN

0162-0134

Periodical

JOURNAL OF INORGANIC BIOCHEMISTRY

Year of study

217

Number

111395

State

United States of America

Pages from

1

Pages to

10

Pages count

10

URL

https://doi.org/10.1016/j.jinorgbio.2021.111395

BibTex

@article{BUT171638,
  author="MASARYK, L. and TESAŘOVÁ, B. and CHOQUESILLO-LAZARTE, D. and MILOSAVLJEVIĆ, V. and HEGER, Z. and KOPEL, P.",
  title="Structural and biological characterization of anticancer nickel(II) bis(benzimidazole) complex",
  journal="JOURNAL OF INORGANIC BIOCHEMISTRY",
  year="2021",
  volume="217",
  number="111395",
  pages="1--10",
  doi="10.1016/j.jinorgbio.2021.111395",
  issn="0162-0134",
  url="https://doi.org/10.1016/j.jinorgbio.2021.111395"
}