Publication detail

Peptide-based electrochemical biosensors utilized for protein detection

VAŇOVÁ, V. MITREVSKA, K. MILOSAVLJEVIĆ, V. HYNEK, D. RICHTERA, L. ADAM, V.

Original Title

Peptide-based electrochemical biosensors utilized for protein detection

Type

journal article in Web of Science

Language

English

Original Abstract

Proteins are generally detected as biomarkers for tracing or determining various disorders in organisms. Biomarker proteins can be tracked in samples with various origins and in different concentrations, revealing whether an organism is in a healthy or unhealthy state. In regard to detection, electrochemical biosensors are a potential fusion of electronics, chemistry, and biology, allowing for fast and early point-of-care detection from a biological sample with the advantages of high sensitivity, simple construction, and easy operation. Peptides present a promising approach as a biorecognition element when connected with electrochemical biosensors. The benefits of short peptides lie mainly in their good stability and selective affinity to a target analyte. Therefore, peptide-based electrochemical biosensors (PBEBs) represent an alternative approach for the detection of different protein biomarkers. This review provides a summary of the past decade of recently proposed PBEBs designed for protein detection, dividing them according to different protein types: (i) enzyme detection, including proteases and kinases; (ii) antibody detection; and (iii) other protein detection. According to these protein types, different sensing mechanisms are discussed, such as the peptide cleavage by a proteases, phosphorylation by kinases, presence of antibodies, and exploiting of affinities; furthermore, measurements are obtained by different electrochemical methods. A discussion and comparison of various constructions, modifications, immobilization strategies and different sensing techniques in terms of high sensitivity, selectivity, repeatability, and potential for practical application are presented.

Keywords

Proteins; Biomarkers; Electrochemistry; Biosensors; Peptides; Biorecognition element

Authors

VAŇOVÁ, V.; MITREVSKA, K.; MILOSAVLJEVIĆ, V.; HYNEK, D.; RICHTERA, L.; ADAM, V.

Released

15. 5. 2021

ISBN

0956-5663

Periodical

BIOSENSORS & BIOELECTRONICS

Year of study

180

Number

113087

State

Kingdom of the Netherlands

Pages from

1

Pages to

19

Pages count

19

URL

https://www.sciencedirect.com/science/article/pii/S095656632100124X

BibTex

@article{BUT171293,
  author="VAŇOVÁ, V. and MITREVSKA, K. and MILOSAVLJEVIĆ, V. and HYNEK, D. and RICHTERA, L. and ADAM, V.",
  title="Peptide-based electrochemical biosensors utilized for protein detection",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2021",
  volume="180",
  number="113087",
  pages="1--19",
  doi="10.1016/j.bios.2021.113087",
  issn="0956-5663",
  url="https://www.sciencedirect.com/science/article/pii/S095656632100124X"
}