Publication detail

LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae

DO, T. JAROŠOVÁ, R. SEDLÁČKOVÁ, E. GURÁŇ, R. ONDRÁČKOVÁ, P. FALDYNA, M. SLÁDEK, Z. ZÍTKA, O.

Original Title

LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae

Type

conference paper

Language

English

Original Abstract

Targeted therapy of porcine pleuropneumonia, which is caused by the bacterium Actinobacillus pleuropneumoniae, would help pig breeders to lower their expenses related with solving this problem. In order to find potential receptors of a targeted therapy it is important to know the response of the organismon an infection and detect proteins connected to an immune reaction. Nowadays, a liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) is one of the most used methods for this purpose. This study was aimed at detection and identification of proteins after homogenization and extraction from the porcine lung tissue cryosections by applying LC-MS/MS method using high-performance liquid chromatography-electrospray ionisation quadrupole-quadrupole time-of-flight mass spectrometry (HPLC-ESI-QqTOF MS).

Keywords

pig; liquid chromatography; tandem mass spectrometry; electrospray-time of flight mass spectrometry; pulmonary infection; proteins

Authors

DO, T.; JAROŠOVÁ, R.; SEDLÁČKOVÁ, E.; GURÁŇ, R.; ONDRÁČKOVÁ, P.; FALDYNA, M.; SLÁDEK, Z.; ZÍTKA, O.

Released

6. 11. 2019

ISBN

978-80-7509-688-3

Book

MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE

Pages from

563

Pages to

568

Pages count

6

BibTex

@inproceedings{BUT170318,
  author="Tomáš {Do} and Rea {Jarošová} and Eliška {Dostálová} and Roman {Guráň} and Petra {Ondráčková} and Martin {Faldyna} and Zbyšek {Sládek} and Ondřej {Zítka}",
  title="LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae",
  booktitle="MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE",
  year="2019",
  pages="563--568",
  isbn="978-80-7509-688-3"
}