Publication detail

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

NĚMČÁKOVÁ, I. BLAHOVÁ, L. RYŠÁNEK, P. BLANQUER, A. BAČÁKOVÁ, L. ZAJÍČKOVÁ, L.

Original Title

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

Type

journal article in Web of Science

Language

English

Original Abstract

Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.

Keywords

amine plasma polymer; bioactive coating; polycaprolactone nanofibers; substrate morphology; cell adhesion; cell proliferation

Authors

NĚMČÁKOVÁ, I.; BLAHOVÁ, L.; RYŠÁNEK, P.; BLANQUER, A.; BAČÁKOVÁ, L.; ZAJÍČKOVÁ, L.

Released

1. 12. 2020

Publisher

MDPI

Location

BASEL

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

21

Number

24

State

Swiss Confederation

Pages from

1

Pages to

24

Pages count

24

URL

https://www.mdpi.com/1422-0067/21/24/9467

Full text in the Digital Library

http://hdl.handle.net/11012/196474

BibTex

@article{BUT169628,
  author="Ivana {Němčáková} and Lucie {Janů} and Petr {Ryšánek} and Andreu {Blanquer} and Lucie {Bačáková} and Lenka {Zajíčková}",
  title="Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies",
  journal="International Journal of Molecular Sciences",
  year="2020",
  volume="21",
  number="24",
  pages="1--24",
  doi="10.3390/ijms21249467",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/21/24/9467"
}