Publication detail

Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections

MAZUMDAR, A. HADDAD, Y. SUR, V. MILOSAVLJEVIĆ, V. BHOWMICK, S. MICHÁLKOVÁ, H. GURÁŇ, R. VESELÝ, R. MOULICK, A.

Original Title

Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections

Type

journal article in Web of Science

Language

English

Original Abstract

An inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.

Keywords

antibacterial peptides; antibiotics; multidrug resistance; bacteria; infections

Authors

MAZUMDAR, A.; HADDAD, Y.; SUR, V.; MILOSAVLJEVIĆ, V.; BHOWMICK, S.; MICHÁLKOVÁ, H.; GURÁŇ, R.; VESELÝ, R.; MOULICK, A.

Released

25. 8. 2020

Publisher

Frontiers Media

ISBN

1664-302X

Periodical

Frontiers in Microbiology

Year of study

11

Number

1

State

Swiss Confederation

Pages from

1

Pages to

19

Pages count

19

URL

https://doi.org/10.3389/fmicb.2020.01963

Full text in the Digital Library

http://hdl.handle.net/11012/196325

BibTex

@article{BUT169138,
  author="Aninda {Mazumdar} and Yazan Abdulmajeed Eyadh {Haddad} and Vishma Pratap {Sur} and Vedran {Milosavljević} and Sukanya {Bhowmick} and Hana {Michálková} and Roman {Guráň} and Radek {Veselý} and Amitava {Moulick}",
  title="Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections",
  journal="Frontiers in Microbiology",
  year="2020",
  volume="11",
  number="1",
  pages="1--19",
  doi="10.3389/fmicb.2020.01963",
  issn="1664-302X",
  url="https://doi.org/10.3389/fmicb.2020.01963"
}