Publication detail

Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay

SEDLÁČKOVÁ, E. BYTEŠNÍKOVÁ, Z. BIRGUSOVÁ, E. ŠVEC, P. AMIRMANSOOR, A. ESTRELA, P. RICHTERA, L.

Original Title

Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay

Type

journal article in Web of Science

Language

English

Original Abstract

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)-gold (AuNPs), silver (AgNPs), and copper (CuNPs)-was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA-1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5 '-CCGG-3 ') islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U center dot mL(-1). Lastly, this study was enriched with the real serum sample, where a 0.19 U center dot mL(-1) LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Keywords

nanomaterials; electrochemical biosensor; DNA methylation; rGO; biomedical applications

Authors

SEDLÁČKOVÁ, E.; BYTEŠNÍKOVÁ, Z.; BIRGUSOVÁ, E.; ŠVEC, P.; AMIRMANSOOR, A.; ESTRELA, P.; RICHTERA, L.

Released

30. 11. 2020

Publisher

MDPI

ISBN

1996-1944

Periodical

Materials

Year of study

13

Number

21

State

Swiss Confederation

Pages from

1

Pages to

12

Pages count

12

URL

https://www.mdpi.com/1996-1944/13/21/4936

Full text in the Digital Library

http://hdl.handle.net/11012/195920

BibTex

@article{BUT168307,
  author="Eliška {Dostálová} and Zuzana {Bytešníková} and Eliška {Birgusová} and Pavel {Švec} and Amirmansoor {Ashrafi} and Pedro {Estrela} and Lukáš {Richtera}",
  title="Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay",
  journal="Materials",
  year="2020",
  volume="13",
  number="21",
  pages="1--12",
  doi="10.3390/ma13214936",
  issn="1996-1944",
  url="https://www.mdpi.com/1996-1944/13/21/4936"
}