Publication detail

MALDI MSI reveals the spatial distribution of protein markers in tracheobronchial lymph nodes and lung of pigs after respiratory infection

DO, T. GURÁŇ, R. JAROŠOVÁ, R. ONDRÁČKOVÁ, P. SLÁDEK, Z. FALDYNA, M. ADAM, V. ZÍTKA, O.

Original Title

MALDI MSI reveals the spatial distribution of protein markers in tracheobronchial lymph nodes and lung of pigs after respiratory infection

Type

journal article in Web of Science

Language

English

Original Abstract

Respiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system-lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1 beta (IL-1 beta) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL-1 beta were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF/TOF directly on the tissue cryosections. CD163, IL-1 beta and protegrin-4 precursor were all significantly (p < 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs.

Keywords

CD163; interleukin 1β; protegrin-4 precursor; MALDI MSI; pig model; lungs infection; Actinobacillus pleuropneumoniae

Authors

DO, T.; GURÁŇ, R.; JAROŠOVÁ, R.; ONDRÁČKOVÁ, P.; SLÁDEK, Z.; FALDYNA, M.; ADAM, V.; ZÍTKA, O.

Released

30. 12. 2020

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

25

Number

5723

State

Swiss Confederation

Pages from

1

Pages to

16

Pages count

16

URL

https://www.mdpi.com/1420-3049/25/23/5723

Full text in the Digital Library

http://hdl.handle.net/11012/195918

BibTex

@article{BUT168281,
  author="Tomáš {Do} and Roman {Guráň} and Rea {Jarošová} and Petra {Ondráčková} and Zbyšek {Sládek} and Martin {Faldyna} and Vojtěch {Adam} and Ondřej {Zítka}",
  title="MALDI MSI reveals the spatial distribution of protein markers in tracheobronchial lymph nodes and lung of pigs after respiratory infection",
  journal="MOLECULES",
  year="2020",
  volume="25",
  number="5723",
  pages="1--16",
  doi="10.3390/molecules25235723",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/25/23/5723"
}