Publication detail

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

ADAMOVSKÝ, O. BOYDA, J. CRAFT, S. GINN, P. HUA, Q. KOZUCH, M. SCHMIDT, J. SMATANA, S. VESPALCOVÁ, H. PERSICO, M. BISESI, J. BUDINSKÁ, E. MARTYNIUK, C.

Original Title

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

Type

journal article in Web of Science

Language

English

Original Abstract

Dietary exposure to chemicals can alter the diversity of microbiome communities and can lead to pathophysiological changes in the gastrointestinal system. The organochlorine pesticide dieldrin is a  persistent environmental contaminant that bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study were to determine whether environmentally-relevant doses of dieldrin altered gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at ~4 months of age were fed a measured amount of feed containing either a solvent control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry weight) for 4 months. Body burden levels of dieldrin in zebrafish at the end of the four-month exposure were 0.600.52 ng/g (background), 11.471.13 ng/g (low dose) and 18.321.32 ng/g (high dose) wet weight [meanstd]. Extensive histopathology at the whole organism level revealed that dieldrin exposure did not induce notable tissue pathology, including the gastrointestinal tract. A repeated measures mixed model revealed that, while fish gained weight over time, there were no dieldrin-specific effects on body mass. Fecal content was collected from the gastrointestinal tract of males and 16S rRNA sequencing conducted. Dieldrin at a dose of 16 ng/g reduced the abundance of Firmicutes, a  phylum involved in energy resorption. At the level of class, there was a decrease in abundance of Clostridia and Betaproteobacteria, and an increase in Verrucomicrobiae species. We used predicted relative metabolomic turnover (PRMA) to predict how a shift in microbial community composition affects exchange of metabolites. Dieldrin was predicted to affect metabolic turnover of uroporphyrinogen I and coproporphyrinogen I [enzyme]cysteine, hydrogen selenide, selenite, and methyl-selenic acid in fish. These pathways are related to bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that dietary exposures to dieldrin can alter microbiota composition, however the long-term consequences of such impacts are not well understood.

Keywords

Microbiome, Pesticide, Aquatic, Ecotoxicology, Pathology

Authors

ADAMOVSKÝ, O.; BOYDA, J.; CRAFT, S.; GINN, P.; HUA, Q.; KOZUCH, M.; SCHMIDT, J.; SMATANA, S.; VESPALCOVÁ, H.; PERSICO, M.; BISESI, J.; BUDINSKÁ, E.; MARTYNIUK, C.

Released

5. 10. 2020

ISBN

1873-6424

Periodical

ENVIRONMENTAL POLLUTION

Year of study

2021

Number

268

State

United Kingdom of Great Britain and Northern Ireland

Pages from

115715

Pages to

115726

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S0269749120364046?casa_token=D-NvNb5oSOkAAAAA:TSekabQnMmwF-daj6ik3X3S1GHcD0DJnPcXtWzs4_hHCGfEXh0ZMGU1t_8Wyome1iZMN2kVeqw

BibTex

@article{BUT168159,
  author="ADAMOVSKÝ, O. and BOYDA, J. and CRAFT, S. and GINN, P. and HUA, Q. and KOZUCH, M. and SCHMIDT, J. and SMATANA, S. and VESPALCOVÁ, H. and PERSICO, M. and BISESI, J. and BUDINSKÁ, E. and MARTYNIUK, C.",
  title="Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin",
  journal="ENVIRONMENTAL POLLUTION",
  year="2020",
  volume="2021",
  number="268",
  pages="115715--115726",
  doi="10.1016/j.envpol.2020.115715",
  issn="1873-6424",
  url="https://www.sciencedirect.com/science/article/pii/S0269749120364046?casa_token=D-NvNb5oSOkAAAAA:TSekabQnMmwF-daj6ik3X3S1GHcD0DJnPcXtWzs4_hHCGfEXh0ZMGU1t_8Wyome1iZMN2kVeqw"
}