Publication detail

Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification

KŘEPELKA, P. PÉREZ-RODRÍGUEZ, F. BOLÍVAR, A.

Original Title

Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification

Type

journal article in Web of Science

Language

English

Original Abstract

In recent years, near infrared (NIR) spectroscopy has gained interest as a tool for bacteria strain identification. Although some promising results suggest good applicability of the technique, a better interpretation of the NIR bacterial spectra is still needed. In order to analyze the NIR spectrum of biological samples, a correlation analysis between the NIR and the mid-infrared (mid-IR) spectra was performed. In total, 28 spectra of 8 bacterial strains were acquired and correlated in the NIR and the mid-IR spectral ranges. Some molecular bands (Amide I, P¼O stretching, C-H stretching/deformation of polysaccharides) were well correlated, and the effect of concentration changes in these molecules were investigated. Moreover, a model for the NIR spectra classification was created with an overall 85% correct classification rate. Subsequently, only NIR wavelengths with high correlation to important mid-IR peaks were selected. This led to an increase in the correct classification rate to 94%. By correlation between well-established mid-IR peaks and NIR spectra, some relationships in the NIR spectra of biological samples were revealed, which was a step towards better understanding and interpretation of the NIR spectra of biological samples.

Keywords

Bacterial identification, classification, near infrared, mid infrared, correlation, 2D correlation, microbiology

Authors

KŘEPELKA, P.; PÉREZ-RODRÍGUEZ, F.; BOLÍVAR, A.

Released

27. 11. 2020

Publisher

SAGE publishing

ISBN

1751-6552

Periodical

JOURNAL OF NEAR INFRARED SPECTROSCOPY

Year of study

1

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

10

Pages count

10

URL

https://journals.sagepub.com/doi/10.1177/0967033520974518

BibTex

@article{BUT166451,
  author="Pavel {Křepelka} and Fernando {Pérez-Rodríguez} and Araceli {Bolívar}",
  title="Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification",
  journal="JOURNAL OF NEAR INFRARED SPECTROSCOPY",
  year="2020",
  volume="1",
  number="1",
  pages="1--10",
  doi="10.1177/0967033520974518",
  issn="1751-6552",
  url="https://journals.sagepub.com/doi/10.1177/0967033520974518"
}