Publication detail

One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

GAGIĆ, M. KOČIOVÁ, S. ŠMERKOVÁ, K. MICHÁLKOVÁ, H. ŠETKA, M. ŠVEC, P. PŘIBYL, J. MÁSILKO, J. BÁLKOVÁ, R. HEGER, Z. RICHTERA, L. ADAM, V. MILOSAVLJEVIĆ, V.

Original Title

One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

Type

journal article in Web of Science

Language

English

Original Abstract

In the present study, the thermal decomposition of citric acid in the presence of biogenic amine was used to synthesize four different functionalized carbon quantum dots (CQDs), namely, histamine-(HCQDs), putrescine-(PCQDs), cadaverine-(CCQDs) and spermine-(SCQDs). The thermal decomposition of the precursors resulted in a decrease in stability and the formation of surface amides via a cross-linking process between the carboxyl and amine groups. The deposition of biogenic amines was confirmed by a structural characterization of the synthesized CQDs. The resulting CQDs, with a net zero charge, exhibited excellent stability in environments with different pH values. Through a set of different cytotoxicity tests, the absence of gene mutations, apoptosis, necrosis or disruption in cell membranes revealed the high biocompatibility of the CQDs. The antimicrobial activity of the synthesized CQDs was investigated against different bacterial species (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia). We determined the growth kinetics, production of reactive oxygen species (ROS), cell viability and changes in membrane integrity by scanning electron microscopy (SEM). The minimal inhibitory concentrations (MICs) for S. aureus ranged from 3.4 to 6.9 mu g/mL. Regarding E. coli and K. pneumonia, all CQD formulations reduced growth, and the MICs were determined for CCQDs and HCQDs (6.9-19.4 mu g/mL). The antibacterial activity mechanism was attributed to the oxidative stress generated after CQD treatment, which resulted in the destabilization of the bacterial membrane. The bacterial permeability to propidium iodide indicated a change in membrane integrity, and the effect of CQDs on the morphology of the bacterial cells was evidenced by SEM.

Keywords

Carbon quantum dots; Amines; Thermal decomposition; Toxicity; Antibacterial activity; ROS

Authors

GAGIĆ, M.; KOČIOVÁ, S.; ŠMERKOVÁ, K.; MICHÁLKOVÁ, H.; ŠETKA, M.; ŠVEC, P.; PŘIBYL, J.; MÁSILKO, J.; BÁLKOVÁ, R.; HEGER, Z.; RICHTERA, L.; ADAM, V.; MILOSAVLJEVIĆ, V.

Released

15. 11. 2020

ISBN

0021-9797

Periodical

Journal of Colloid and Interface Science

Year of study

580

Number

1

State

United States of America

Pages from

30

Pages to

48

Pages count

19

URL

https://doi.org/10.1016/j.jcis.2020.06.125

BibTex

@article{BUT166168,
  author="Milica {Gagić} and Silvia {Kočiová} and Kristýna {Šmerková} and Hana {Michálková} and Milena {Šetka} and Pavel {Švec} and Jan {Přibyl} and Jiří {Másilko} and Radka {Bálková} and Zbyněk {Heger} and Lukáš {Richtera} and Vojtěch {Adam} and Vedran {Milosavljević}",
  title="One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity",
  journal="Journal of Colloid and Interface Science",
  year="2020",
  volume="580",
  number="1",
  pages="30--48",
  doi="10.1016/j.jcis.2020.06.125",
  issn="0021-9797",
  url="https://doi.org/10.1016/j.jcis.2020.06.125"
}