Publication detail

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

BEZDĚKOVÁ, J. ZEMÁNKOVÁ, K. HUTAŘOVÁ, J. KOČIOVÁ, S. ŠMERKOVÁ, K. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Type

journal article in Web of Science

Language

English

Original Abstract

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 x 10(3) CFU.ml(-1), which corresponds to the limit set in European Union legislation for microbial control of food.

Keywords

Molecularly imprinted polymers; Magnetic particles; Dopamine; Bacteria; Fluorescence microscopy

Authors

BEZDĚKOVÁ, J.; ZEMÁNKOVÁ, K.; HUTAŘOVÁ, J.; KOČIOVÁ, S.; ŠMERKOVÁ, K.; ADAM, V.; VACULOVIČOVÁ, M.

Released

15. 8. 2020

ISBN

0308-8146

Periodical

FOOD CHEMISTRY

Year of study

321

Number

126673

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

8

Pages count

8

URL

https://www.sciencedirect.com/science/article/abs/pii/S0308814620305355

BibTex

@article{BUT164091,
  author="Jaroslava {Bezděková} and Kristýna {Zemánková} and Jitka {Hutařová} and Silvia {Kočiová} and Kristýna {Šmerková} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus",
  journal="FOOD CHEMISTRY",
  year="2020",
  volume="321",
  number="126673",
  pages="1--8",
  doi="10.1016/j.foodchem.2020.126673",
  issn="0308-8146",
  url="https://www.sciencedirect.com/science/article/abs/pii/S0308814620305355"
}