Publication detail

New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures

PUENTE-MUNOZ, V. PAREDES, J., M. Resa, S. Vilchez, J., D. Žitňan, M. Miguel, D. Giron, M., D. Cuerva, J., M. Salto, R. Crovetto, L.

Original Title

New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures

Type

journal article in Web of Science

Language

English

Original Abstract

A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.

Keywords

molecular biology, chemistry

Authors

PUENTE-MUNOZ, V.; PAREDES, J., M.; Resa, S.; Vilchez, J., D.; Žitňan, M.; Miguel, D.; Giron, M., D.; Cuerva, J., M.; Salto, R.; Crovetto, L.

Released

7. 2. 2019

Publisher

NATURE PUBLISHING GROUP

Location

LONDON

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

9

Number

2019

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1659

Pages to

1669

Pages count

10

URL

https://www.nature.com/articles/s41598-018-38132-y

BibTex

@article{BUT161966,
  author="PUENTE-MUNOZ, V. and PAREDES, J., M. and Resa, S. and Vilchez, J., D. and Žitňan, M. and Miguel, D. and Giron, M., D. and Cuerva, J., M. and Salto, R. and Crovetto, L.",
  title="New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="2019",
  pages="1659--1669",
  doi="10.1038/s41598-018-38132-y",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-018-38132-y"
}