Publication detail

Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

JAROŠOVÁ, R. SMOLÍKOVÁ, V. DVOŘÁK, M. GURÁŇ, R. DO, T. ONDRÁČKOVÁ, P. ZÍTKA, O. SLÁDEK, Z.

Original Title

Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Type

conference paper

Language

English

Original Abstract

In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.

Keywords

cryosectioning; lungs; pig; pleuropneumonia; mass spectrometry imaging

Authors

JAROŠOVÁ, R.; SMOLÍKOVÁ, V.; DVOŘÁK, M.; GURÁŇ, R.; DO, T.; ONDRÁČKOVÁ, P.; ZÍTKA, O.; SLÁDEK, Z.

Released

2. 1. 2019

Publisher

Mendel University in Brno

ISBN

978-80-7509-597-8

Book

MendelNet 2018

Pages from

506

Pages to

511

Pages count

6

URL

https://mendelnet.cz/artkey/mnt-201801-0105_Preparation-of-cryosections-from-frozen-porcine-pulmonary-tissue-for-MALDI-mass-spectrometry-imaging.php?back=/magno/mnt/2018/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/178383

BibTex

@inproceedings{BUT156237,
  author="Rea {Jarošová} and Vendula {Smolíková} and Marek {Dvořák} and Roman {Guráň} and Tomáš {Do} and Petra {Ondráčková} and Ondřej {Zítka} and Zbyšek {Sládek}",
  title="Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging",
  booktitle="MendelNet 2018",
  year="2019",
  pages="506--511",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-597-8",
  url="https://mendelnet.cz/artkey/mnt-201801-0105_Preparation-of-cryosections-from-frozen-porcine-pulmonary-tissue-for-MALDI-mass-spectrometry-imaging.php?back=/magno/mnt/2018/mn1.php?secid=4"
}