Publication detail

Microstructure, chemical and biological performance of boron-modified TiCaPCON films

PONOMAREV, V. SHEVEYKO, A. SUKHORUKOVA, I. SHVINDINA, N. MANAKHOV, A. ZHITNYAK, I. GLOUSHANKOVA, N. FURSOVA, N. IGNATOV, S. PERMYAKOVA, E. POLČÁK, J. SHTANSKY, D.

Original Title

Microstructure, chemical and biological performance of boron-modified TiCaPCON films

Type

journal article in Web of Science

Language

English

Original Abstract

The fabrication of biologically safe materials with enhanced antibacterial characteristics is of great significance. Here we propose B-based therapeutic strategy which offers a good alternative to the widely used approach based on the introduction of most famous bactericidal elements, such as Ag, Cu, and Zn. B-doped TiCaPCON films with 8, 11, and 15 at.% of B were obtained by simultaneous magnetron sputtering of two composite targets (TiC-CaOTi3POx and TiB2) in a gaseous mixture of Ar+15%N2. The B content in the films was controlled by changing magnetron current of the TiB2 target. The B-doped films were thoroughly studied by means of scanning and transmission electron microscopy, high-resolution TEM, X-ray diffraction, Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and glow discharge optical emission spectroscopy. The films were also characterized with respect to surface wettability, boron ion release, and electrochemical characteristics. To test biocompatibility and bioactivity of the films in vitro, spreading, actin cytoskeleton organization, proliferation and osteogenic differentiation of MC3T3-E1 osteoblastic cells grown on the film surface were studied. The antibacterial characteristics of the B-doped TiCaPCON samples against antibiotic- resistant Escherichia coli (E. coli) 261 strains were compared with two-layer BOx/TiCaPCON–B and TiOx/ TiCaPCON–B films obtained by three different methods: (i) deposition of a B2O3 top layer, (ii) annealing and (iii) electrochemical oxidation of the as-deposited B-doped films. The obtained results clearly indicated that all types of B-containing films are not toxic for cells regardless of their surface composition. Antibacterial activity tests revealed that only the sample with B2O3 top layer completely inactivated E. coli cells and prevented biofilm formation. Thus the surface of two-layer B2O3/TiCaPCON–11%B film was simultaneously bactericidal towards E. coli strains and not-toxic for osteoblastic cells.

Keywords

Magnetron sputtering B-doped TiCaPCON films Microstructural characterization Biocompatibility Antibacterial activity

Authors

PONOMAREV, V.; SHEVEYKO, A.; SUKHORUKOVA, I.; SHVINDINA, N.; MANAKHOV, A.; ZHITNYAK, I.; GLOUSHANKOVA, N.; FURSOVA, N.; IGNATOV, S.; PERMYAKOVA, E.; POLČÁK, J.; SHTANSKY, D.

Released

28. 1. 2019

ISBN

0169-4332

Periodical

Applied Surface Science

Year of study

465

Number

1

State

Kingdom of the Netherlands

Pages from

486

Pages to

497

Pages count

12

URL

https://www.sciencedirect.com/science/article/pii/S016943321832573X

BibTex

@article{BUT152109,
  author="Viktor A. {Ponomarev} and A.N. {Sheveyko} and Irina V. {Sukhorukova} and Natalia V. {Shvindina} and Anton {Manakhov} and I.Y. {Zhitnyak} and Natalia A. {Gloushankova} and Nadezda K. {Fursova} and Sergei G. {Ignatov} and Elizaveta {Permyakova} and Josef {Polčák} and Dmitry V. {Shtansky}",
  title="Microstructure, chemical and biological performance of boron-modified TiCaPCON films",
  journal="Applied Surface Science",
  year="2019",
  volume="465",
  number="1",
  pages="486--497",
  doi="10.1016/j.apsusc.2018.09.157",
  issn="0169-4332",
  url="https://www.sciencedirect.com/science/article/pii/S016943321832573X"
}