Publication detail

BIOCONVERSION OF LOW-COST FAT MATERIALS INTO HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS

M. Vysoká, M. Szotkowski, K. Bradáčová, J. Holub, P. Matoušková, S. Šimanský, M. Čertík, I. Márová

Original Title

BIOCONVERSION OF LOW-COST FAT MATERIALS INTO HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS

English Title

BIOCONVERSION OF LOW-COST FAT MATERIALS INTO HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS

Type

abstract

Language

en

Original Abstract

Carotenoids are natural pigments occurring in plants and many microorganisms, such as algae, yeast and bacteria. Carotenoid yeasts are organisms capable of producing large amounts of biomass, enriched with mono and polyunsaturated fatty acids, with the use of lowcost waste substrates. Due to their antioxidant properties or antitumor and cancer-preventative action in cell lines properties, carotenoids find use in the food, agricultural or pharmaceutical industries. Lipids are used as biofuels and polyunsaturated fatty acids have a positive effect on human health, saturated fatty acids are therefore primarily a source of energy. Carotenoids, coenzyme Q and ergosterol in cells were determined by HPLC/PDA/MS. The lipid content and fatty acid profiles were analyzed by gas chromatography (GC/FID). Yeasts were cultivated on media with different carbon sources – glucose, glycerol and waste fat material from livestock production. For yeast cultivation, fat was used in its pure form or after basic, acidic or enzymatic hydrolysis. In addition, the use of various emulsifiers (Tween, PEG 400, Fatty acid salts) was tested. For these experiments Cystofilobasidium, Sporobolomycetes and Rhodotorula strains were used. The results showed that enzymatically hydrolyzed fat seems to be appropriate source for production of high value biomass. Method of base hydrolyzes proved to be the most efficient for carotenoid production. The composition of cultivation media and cultivation conditions should be further optimized.

English abstract

Carotenoids are natural pigments occurring in plants and many microorganisms, such as algae, yeast and bacteria. Carotenoid yeasts are organisms capable of producing large amounts of biomass, enriched with mono and polyunsaturated fatty acids, with the use of lowcost waste substrates. Due to their antioxidant properties or antitumor and cancer-preventative action in cell lines properties, carotenoids find use in the food, agricultural or pharmaceutical industries. Lipids are used as biofuels and polyunsaturated fatty acids have a positive effect on human health, saturated fatty acids are therefore primarily a source of energy. Carotenoids, coenzyme Q and ergosterol in cells were determined by HPLC/PDA/MS. The lipid content and fatty acid profiles were analyzed by gas chromatography (GC/FID). Yeasts were cultivated on media with different carbon sources – glucose, glycerol and waste fat material from livestock production. For yeast cultivation, fat was used in its pure form or after basic, acidic or enzymatic hydrolysis. In addition, the use of various emulsifiers (Tween, PEG 400, Fatty acid salts) was tested. For these experiments Cystofilobasidium, Sporobolomycetes and Rhodotorula strains were used. The results showed that enzymatically hydrolyzed fat seems to be appropriate source for production of high value biomass. Method of base hydrolyzes proved to be the most efficient for carotenoid production. The composition of cultivation media and cultivation conditions should be further optimized.

Keywords

Carotenoids, Lipides, Yeast, Cultivation

Released

18.05.2018

Pages from

64

Pages to

64

Pages count

79

URL

BibTex


@misc{BUT149002,
  author="Petra {Matoušková} and Marie {Vysoká} and Ivana {Márová} and Martin {Szotkowski} and Kristína {Bradáčová} and Samuel {Šimanský} and Milan {Čertík} and Jiří {Holub}",
  title="BIOCONVERSION OF LOW-COST FAT MATERIALS INTO
HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS",
  annote="Carotenoids are natural pigments occurring in plants and many microorganisms, such
as algae, yeast and bacteria. Carotenoid yeasts are organisms capable of producing large
amounts of biomass, enriched with mono and polyunsaturated fatty acids, with the use of lowcost
waste substrates. Due to their antioxidant properties or antitumor and cancer-preventative
action in cell lines properties, carotenoids find use in the food, agricultural or pharmaceutical
industries. Lipids are used as biofuels and polyunsaturated fatty acids have a positive effect on
human health, saturated fatty acids are therefore primarily a source of energy.
Carotenoids, coenzyme Q and ergosterol in cells were determined by HPLC/PDA/MS.
The lipid content and fatty acid profiles were analyzed by gas chromatography (GC/FID). Yeasts
were cultivated on media with different carbon sources – glucose, glycerol and waste fat
material from livestock production. For yeast cultivation, fat was used in its pure form or after
basic, acidic or enzymatic hydrolysis. In addition, the use of various emulsifiers (Tween, PEG
400, Fatty acid salts) was tested. For these experiments Cystofilobasidium, Sporobolomycetes
and Rhodotorula strains were used.
The results showed that enzymatically hydrolyzed fat seems to be appropriate source
for production of high value biomass. Method of base hydrolyzes proved to be the most efficient
for carotenoid production. The composition of cultivation media and cultivation conditions should
be further optimized.",
  chapter="149002",
  howpublished="online",
  year="2018",
  month="may",
  pages="64--64",
  type="abstract"
}