Publication detail

Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation

HUDEČKOVÁ, H. ŠUPINOVÁ, P. BABÁK, L.

Original Title

Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation

Type

journal article in Scopus

Language

English

Original Abstract

Finding of optimal hydrolysis conditions is important for increasing the yield of saccharides. The higher yield of saccharides is usable for increase of the following fermentation effectivity. In this study optimal conditions (pH and temperature) for amylolytic enzymes were searched. As raw material was used waste bread. Two analytical methods for analysis were used. Efficiency and process of hydrolysis was analysed spectrophotometrically by Somogyi-Nelson method. Final yields of glucose were analysed by HPLC. As raw material was used waste bread from local cafe. Waste bread was pretreated by grinding into small particles. Hydrolysis was performed in 100 mL of 15 % (w/v) waste bread particles in the form of water suspension. Waste bread was hydrolysed by two commercial enzymes. For the liquefaction was used α‑amylase (BAN 240 L). The saccharification was performed by glucoamylase (AMG 300 L). Optimal conditions for α‑amylase (pH 6; 80 °C) were found. The yield of total sugars was 67.08 g∙L-1 (calculated to maltose). As optimal conditions for glucoamylase (pH 4.2; 60 °C) were found. Amount of glucose was 70.28 g∙L1. The time of waste bread liquefaction was 180 minutes. The time of saccharification was 90 minutes. The results were presented at the conference CECE Junior 2014.

Keywords

ethanol, waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature

Authors

HUDEČKOVÁ, H.; ŠUPINOVÁ, P.; BABÁK, L.

Released

28. 2. 2017

Publisher

Mendel University Press

Location

Brno

ISBN

1211-8516

Periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

Year of study

65

Number

1

State

Czech Republic

Pages from

35

Pages to

40

Pages count

6

URL

https://acta.mendelu.cz/65/1/0035/

Full text in the Digital Library

http://hdl.handle.net/11012/68379

BibTex

@article{BUT133640,
  author="Helena {Hudečková} and Petra {Šupinová} and Libor {Babák}",
  title="Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation",
  journal="Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis",
  year="2017",
  volume="65",
  number="1",
  pages="35--40",
  doi="10.11118/actaun201765010035",
  issn="1211-8516",
  url="https://acta.mendelu.cz/65/1/0035/"
}