Publication detail

Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR

Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný

Original Title

Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR

Type

abstract

Language

English

Original Abstract

Real-time polymerase chain reaction (PCR) has become the preferred method for studying expression. The study of gene expression profiles of whole genome is commonly performed by relying on such technique like cDNA microarray. Real-time PCR technique is commonly used to study expression of tens of genes. Real-time PCR is considered to be the most accurate, because of its high sensitivity and specificity. However to validate obtained data reference gene is needed. We have studied gene expression of bacterial strains of Streptococcus equi subsp. zooepidemicus (SEZ).

Keywords

Real-Time PCR, reference gene, gene expression stability

Authors

Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný

Released

3. 12. 2015

Publisher

Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200

Location

Brno

ISBN

978-80-214-5290-9

Book

Sborník abstraktů

Edition

1

Edition number

1

Pages from

65

Pages to

65

Pages count

1

BibTex

@misc{BUT121168,
  author="Radka {Hrudíková}",
  title="Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR",
  booktitle="Sborník abstraktů",
  year="2015",
  series="1",
  edition="1",
  pages="65--65",
  publisher="Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200",
  address="Brno",
  isbn="978-80-214-5290-9",
  note="abstract"
}