Publication detail

Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization

MILOSAVLJEVIĆ, V. NGUYEN, H. MICHÁLEK, P. MOULICK, A. KOPEL, P. KIZEK, R. ADAM, V.

Original Title

Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization

Type

journal article in Web of Science

Language

English

Original Abstract

Nanoparticles as a progressively developing branch offer a tool for studying the interaction of carbon quantum dots (CQDs) with DNA. In this study, fluorescent CQDs were synthesized using citric acid covered with polyethylene glycol (PEG) as the source of carbon precursors. Furthermore, interactions between CQDs and DNA (double-stranded DNA and single-stranded DNA) were investigated by spectral methods, gel electrophoresis, and electrochemical analysis. Primarily, the fluorescent behavior of CQDs in the presence of DNA was monitored and major differences in the interaction of CQDs with tested single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) were observed at different amounts of CQDs (μg mL−1: 25, 50, 100, 250, 500). It was found that the interaction of ssDNA with CQDs had no significant influence on the CQDs fluorescence intensity measured at the excitation wavelengths of 280 nm, 350 nm, and 400 nm. However, in the presence of dsDNA, the fluorescence intensity of CQDs was significantly increased. Our results provide basic understanding of the interaction between CQDs and DNA. Such fabricated CQDs–DNA might be of great benefit

Keywords

carbon quantum dots, DNA, spectrophotometry, gel electrophoresis, electrochemical analysis

Authors

MILOSAVLJEVIĆ, V.; NGUYEN, H.; MICHÁLEK, P.; MOULICK, A.; KOPEL, P.; KIZEK, R.; ADAM, V.

RIV year

2015

Released

1. 1. 2015

ISBN

0366-6352

Periodical

Chemical Papers

Year of study

69

Number

1

State

Slovak Republic

Pages from

192

Pages to

201

Pages count

10

BibTex

@article{BUT121158,
  author="Vedran {Milosavljević} and Hoai Viet {Nguyen} and Petr {Michálek} and Amitava {Moulick} and Pavel {Kopel} and René {Kizek} and Vojtěch {Adam}",
  title="Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization",
  journal="Chemical Papers",
  year="2015",
  volume="69",
  number="1",
  pages="192--201",
  doi="10.2478/s11696-014-0590-2",
  issn="0366-6352"
}