Publication detail

Influence of Carrier Area on Properties of Immobilized Cellulase Complex

REICHSTÄDTER, M. OMELKOVÁ, J. VALICOVÁ, M.

Original Title

Influence of Carrier Area on Properties of Immobilized Cellulase Complex

Type

abstract

Language

English

Original Abstract

Commercial cellulase preparation Celluclast 1.5 L was attached to the synthetic carrier from polyethylene terephtalate (PET) of different surface area, when applying the activation with glutaraldehyde. It was found that all carriers used have always bound the entire amount of the enzyme. The highest relative activity have retained the preparation bound to the carrier with the largest particle size. During the one month storage at 4 °C this preparation showed no decrease in activity. After the immobilization no change of the pH optimum was observed, when compared to the free enzyme. Simultaneously, any change of the any change of the temperature optimum was not proved in comparison to the free enzyme. From experiments, in which the decrease of viscosity of the polymeric substrate as a function of the percentage of cleaved glycosidic linkages was observed, follow that the immobilization alone does not substantially change the mode of action of the immobilized preparation.

Keywords

cellulase; immobilization; polyethylene terephtalate

Authors

REICHSTÄDTER, M.; OMELKOVÁ, J.; VALICOVÁ, M.

Released

4. 9. 2015

Publisher

Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, CZ-612 00 Brno

Location

Brno

ISBN

978-80-214-5228-2

Book

Chemistry & Life 2015 - Book of abstracts

Edition

1

Edition number

1

Pages from

42

Pages to

42

Pages count

1

BibTex

@misc{BUT116526,
  author="Marek {Reichstädter} and Jiřina {Omelková} and Markéta {Valicová}",
  title="Influence of Carrier Area on Properties of Immobilized Cellulase Complex",
  booktitle="Chemistry & Life 2015 - Book of abstracts",
  year="2015",
  series="1",
  edition="1",
  pages="42--42",
  publisher="Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, CZ-612 00 Brno",
  address="Brno",
  isbn="978-80-214-5228-2",
  note="abstract"
}