Publication detail

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

BALVAN, J. KŘÍŽOVÁ, A. GUMULEC, J. RAUDENSKÁ, M. SLÁDEK, Z. SEDLÁČKOVÁ, M. BABULA, P. SZTALMACHOVÁ, M. KIZEK, R. CHMELÍK, R. MASAŘÍK, M.

Original Title

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Type

journal article in Web of Science

Language

English

Original Abstract

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.

Keywords

Multimodal holographic microscopy; apoptosis; oncosis; necrosis; cell death; plumbagin; flow cytometry; transmission electron microscopy

Authors

BALVAN, J.; KŘÍŽOVÁ, A.; GUMULEC, J.; RAUDENSKÁ, M.; SLÁDEK, Z.; SEDLÁČKOVÁ, M.; BABULA, P.; SZTALMACHOVÁ, M.; KIZEK, R.; CHMELÍK, R.; MASAŘÍK, M.

RIV year

2015

Released

24. 3. 2015

Publisher

PLOS

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

10

Number

3

State

United States of America

Pages from

1

Pages to

16

Pages count

16

URL

https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674

Full text in the Digital Library

http://hdl.handle.net/11012/193259

BibTex

@article{BUT113695,
  author="Jan {Balvan} and Aneta {Křížová} and Jaromír {Gumulec} and Martina {Raudenská} and Zbyšek {Sládek} and Miroslava {Sedláčková} and Petr {Babula} and Markéta {Svobodová} and René {Kizek} and Radim {Chmelík} and Michal {Masařík}",
  title="Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis",
  journal="PLOS ONE",
  year="2015",
  volume="10",
  number="3",
  pages="1--16",
  doi="10.1371/journal.pone.0121674",
  issn="1932-6203",
  url="https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674"
}