Publication detail

Chemical and Spectroscopic Characterization of Humic Substances Isolated from South-Bohemian Peat

ENEV, V. NOVÁK, F. KLUČÁKOVÁ, M.

Original Title

Chemical and Spectroscopic Characterization of Humic Substances Isolated from South-Bohemian Peat

English Title

Chemical and Spectroscopic Characterization of Humic Substances Isolated from South-Bohemian Peat

Type

abstract

Language

en

Original Abstract

The aim of this work was study chemical composition and structure of different HS. Object of our study were two samples HS which were isolated from South-Bohemian peat from the quarry Branná near Třeboň, Czech Republic. Isolation of HS was performed according to the procedure recommended by the IHSS. All samples of HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of HS were calculated from the absorbance values. Fluorescence coefficients (Milori index and HIX) of HS were calculated from the area of the emission spectra. Biological/autochthonous index (BIX) and fluorescence index (FI) of HS were calculated from the ratio of different emission intensities. Our results showed that the chemical properties of the HS from South-Bohemian peat used in these experiments were well described using seven complementary indexes derived from the ultraviolet-visible and fluorescence spectra (EET/EBz, E250/E365, E465/E665, FI, BIX, Milori index and HIX). Peat HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. Fulvic acid was characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.

English abstract

The aim of this work was study chemical composition and structure of different HS. Object of our study were two samples HS which were isolated from South-Bohemian peat from the quarry Branná near Třeboň, Czech Republic. Isolation of HS was performed according to the procedure recommended by the IHSS. All samples of HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of HS were calculated from the absorbance values. Fluorescence coefficients (Milori index and HIX) of HS were calculated from the area of the emission spectra. Biological/autochthonous index (BIX) and fluorescence index (FI) of HS were calculated from the ratio of different emission intensities. Our results showed that the chemical properties of the HS from South-Bohemian peat used in these experiments were well described using seven complementary indexes derived from the ultraviolet-visible and fluorescence spectra (EET/EBz, E250/E365, E465/E665, FI, BIX, Milori index and HIX). Peat HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. Fulvic acid was characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.

Keywords

humic substances, UV/Vis, FTIR spectroscopy, fluorescence spectroscopy, absorption and fluorescence coefficients

Released

01.09.2014

Publisher

Printing Office, University of Ioannina

Location

Ioannina, Greece

Pages from

226

Pages to

227

Pages count

2

Documents

BibTex


@misc{BUT109307,
  author="Vojtěch {Enev} and František {Novák} and Martina {Klučáková}",
  title="Chemical and Spectroscopic Characterization of Humic Substances Isolated from South-Bohemian Peat",
  annote="The aim of this work was study chemical composition and structure of different HS. Object of our study were two samples HS which were isolated from South-Bohemian peat from the quarry Branná near Třeboň, Czech Republic. Isolation of HS was performed according to the procedure recommended by the IHSS. All samples of HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of HS were calculated from the absorbance values. Fluorescence coefficients (Milori index and HIX) of HS were calculated from the area of the emission spectra. Biological/autochthonous index (BIX) and fluorescence index (FI) of HS were calculated from the ratio of different emission intensities. Our results showed that the chemical properties of the HS from South-Bohemian peat used in these experiments were well described using seven complementary indexes derived from the ultraviolet-visible and fluorescence spectra (EET/EBz, E250/E365, E465/E665, FI, BIX, Milori index and HIX). Peat HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. Fulvic acid was characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.",
  address="Printing Office, University of Ioannina",
  booktitle="Book of Abstracts: Natural Organic Matter: Structure-Dynamics Innovative Applications",
  chapter="109307",
  howpublished="electronic, physical medium",
  institution="Printing Office, University of Ioannina",
  year="2014",
  month="september",
  pages="226--227",
  publisher="Printing Office, University of Ioannina",
  type="abstract"
}