Publication detail

SPE and purification of DNA using magnetic particles

RITTICH, B., ŠPANOVÁ, A.

Original Title

SPE and purification of DNA using magnetic particles

Type

journal article - other

Language

English

Original Abstract

Superparamagnetic particles have been attractive for molecular diagnostics and analytical chemistry applications due to their unique magnetic properties and their ability to interact with various biomolecules of interest. This paper presents a critical overview of magnetic nano- and microparticles used as a solid phase for extraction and purification of DNAs. The mechanisms of DNA binding to the surface of functionalised magnetic particles are described. The most widely used materials including silica supports, organic polymers and other materials, mostly containing magnetite or paramagnetic metallic elements are reviewed. The main application areas of magnetic particles for DNA separation are briefly described

Keywords

Core covering, DNA isolation, DNA pre-concentration, Magnetic particles, Mechanisms

Authors

RITTICH, B., ŠPANOVÁ, A.

RIV year

2013

Released

30. 8. 2013

Publisher

WILEY-VCH Verlag

Location

Německo

ISBN

1615-9306

Periodical

Journal of Separation Science

Year of study

36

Number

15

State

Federal Republic of Germany

Pages from

2472

Pages to

2485

Pages count

14

BibTex

@article{BUT101526,
  author="Bohuslav {Rittich} and Alena {Španová}",
  title="SPE and purification of DNA using magnetic particles",
  journal="Journal of Separation Science",
  year="2013",
  volume="36",
  number="15",
  pages="2472--2485",
  issn="1615-9306"
}